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Cd166 fitc

Manufactured by BD
Sourced in United States

CD166-FITC is a fluorescently-labeled monoclonal antibody that binds to the CD166 cell surface antigen. CD166 is also known as ALCAM (Activated Leukocyte Cell Adhesion Molecule). The FITC (Fluorescein Isothiocyanate) label allows for the detection and identification of cells expressing CD166 using flow cytometry or other fluorescence-based methods.

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2 protocols using cd166 fitc

1

CD166-FITC Tumor Sphere Characterization

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Tumor spheres were expanded by enzymatic digestion of spheres with Accumax 1X (Innovative Cell Technologies, USA). Single-cells were incubated in staining solution containing 1% BSA with the specific antibodies at appropriate dilutions. Cells were stained with primary conjugated CD166-FITC (BD Biosciences, USA) antibodies, or corresponding isotype-matched controls. Fluorescence-activated cell sorting (FASC) was performed on cells stained with CD166-FITC (BD Biosciences, USA). Quality of sorting was monitored by flow cytometry with specific antibody for CD166-FITC.
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2

Multiparametric Phenotyping of Stem Cell Populations

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Fluorescein isothiocyanate- (FITC-) conjugated CD14, phycoerythrin- (PE-) conjugated CD29, CD31-FITC, peridinin chlorophyll protein- (PerCP-) cyanine (Cy)5.5-conjugated CD34, CD44-FITC, CD45-FITC, CD73-PE, CD90-FITC, CD105-PE, CD166-FITC, CD326-PerCP-Cy5.5, Alexa488-conjugated human leukocyte antigen- (HLA-) ABC, and HLA DR-PE were used (BD Biosciences, Franklin Lakes, NJ; https://www.bdbiosciences.com). Pluripotent embryonic markers NANOG/PE, octamer-binding transcription factor 3/4 (Oct3/4)/PE, stage-specific embryonic antigen (SSEA4)/FITC, and sex-determining region Y-box 2 (SOX2)/FITC were purchased from BD Biosciences.
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