Stool samples were plated on eosin methylene blue agar (Liofilchem, Italy), and the plates were incubated at +37 °C for 18–24 h. After incubation, the suspected colonies were selected and streaked onto Mueller Hinton agar plate (Liofilchem, Italy). Confirmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Liofilchem, Italy), positive indole (Bio-Rad, France), positive lactose (Liofilchem, Italy), and positive orthonitrophenyl-β-D-galactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were confirmed by API 20E (bioMérieux, France).
Urease
Manufactured by Bio-Rad
Sourced in France
Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. It is a commonly used component in various laboratory procedures and assays.
Automatically generated - may contain errors
3 protocols using urease
1
Identification of E. coli from Stool Samples
2
Isolation and Identification of E. coli Pathogroups
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Stool samples were plated on eosin methylene blue agar (Liofilchem, Italy), and the plates were incubated at +37°C for 18-24 h. After incubation, the suspected colonies were selected and streaked onto Mueller Hinton agar plate (Liofilchem, Italy). Confirmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Liofilchem, Italy), positive indole (Bio-Rad, France), positive lactose (Liofi lchem, Italy), and positive orthonitrophenyl-β-Dgalactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were confirmed by API 20E (bioMérieux, France).
The 16-plex PCR was used to detect simultaneously 16 genes from the five main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. 15 . The genes investigated and primers used are listed in Table 1 [15] [16] [17] .
The 16-plex PCR was used to detect simultaneously 16 genes from the five main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. 15 . The genes investigated and primers used are listed in Table 1 [15] [16] [17] .
3
Detection of Pathogenic E. coli using 16-plex PCR
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Stool samples were plated on eosin methylene blue agar (Lio lchem, Italy), and the plates were incubated at + 37 °C for 18-24 h. After incubation, the suspected colonies were selected and streaked onto Mueller Hinton agar plate (Lio lchem, Italy). Con rmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Lio lchem, Italy), positive indole (Bio-Rad, France), positive lactose (Lio lchem, Italy), and positive orthonitrophenyl-β-D-galactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were con rmed by API 20E (bioMérieux, France).
The 16-plex PCR was used to detect simultaneously 16 genes from the ve main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. [15] (link). The genes investigated and primers used are listed in Table 1 [15] (link)[16] [17] (link).
The 16-plex PCR was used to detect simultaneously 16 genes from the ve main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. [15] (link). The genes investigated and primers used are listed in Table 1 [15] (link)[16] [17] (link).
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