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Milli q reverse osmosis system

Manufactured by Merck Group
Sourced in United States

The Milli-Q reverse osmosis system is a water purification device designed to produce high-quality, ultrapure water. It utilizes a reverse osmosis process to remove impurities, dissolved salts, and other contaminants from the input water source. The system is capable of producing water with a high degree of purity, making it suitable for use in a variety of laboratory and research applications.

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4 protocols using milli q reverse osmosis system

1

Quantification of Quercetin by LC-MS

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LC–MS grade mEthanol (> 99.9%) and formic acid (98–100%) were provided by Merck KGaA (Prague, Czech Republic). Ethanol (> 99.7%) was purchased from VWR International (Prague, Czech Republic). Carbon dioxide (> 99.995% purity) was obtained from Messer (Hradec Králové, Czech Republic). Ultrapure water was acquired from the Milli-Q reverse osmosis system (Millipore, Bedford, MA, USA) immediately before use.
Reference standard of quercetin (purity 98.02%) was purchased from MedChemExpress (Sollentuna, Sweden). SIL-IS quercetin-d3 was purchased from Toronto Research Chemicals (Toronto, Ontario, Canada).
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2

Quantification of Fluopyram Pesticide

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The fluopyram standard was purchased from the Environmental Protection Monitoring Institute of the Ministry of Agriculture of China at a concentration of 1000 mg/L. Analytical grade acetonitrile, acetone, dichloromethane, and sodium chloride were purchased from the Guangzhou Chemical Reagent Factory. Chromatographic grade Methanol and n-hexane were available from Thermo Fisher Scientific. Purified water was prepared using a Milli-Q reverse osmosis system (Millipore, Milford, MA, USA). Strata Florisil (FL-PR) 500 mg/6 mL SPE manufactured by Strata™ (5.0 mL n-hexane–acetone (9:1, V/V) solution pre-rinsing cartridge).
A standard solution of 1000 μg/mL fluopyram was diluted in n-hexane, and the matrix extract of the blank sample was obtained by the extraction method. The matrix standard solutions of 0.025, 0.05, 0.10, 0.15 and 0.50 μg/mL were obtained by the step dilution. All prepared solutions were stored at temperature of 4 °C until further use.
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3

Microalgae and Acetobacter Cultivation

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Microalgae cultures were grown in the tris-acetate-phosphate (TAP) culture medium at an incubation temperature of 30 1C. The culture medium of C. reinhardtii consisted of TAP salts O, all purchased from Sigma-Aldrich, UK). Acetobacter cultures were grown in glucose/yeast extract (GY) media at an incubation temperature of 28 1C. The acetobacter growth media consist of glucose purchased from Fisher Scientific, UK and yeast extract purchased from Oxoid Ltd, UK. The fluorescein diacetate (FDA) assay used to determine the viability of the C. reinhardtii cell was purchased from Sigma Aldrich, UK. Deionised water produced using a Milli-Q reverse osmosis system (Millipore, UK) was used in all experiments.
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4

Tissue Engineering Scaffold Fabrication

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Chitosan (high molecular weight), agarose (low melting point) and gelatine were purchased from Sigma-Aldrich; cell culture media and buffers (DMEM, RPMI, PBS, antibiotics) were obtained from Gibco, and foetal bovine serum (FBS) was purchased from Invitrogen. Halloysite nanotube powder was a gift from Applied Minerals Inc., USA. All chemicals were used without further purification. Ultrapure water purified using the Millipore MilliQ reverse osmosis system was used throughout.
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