Airyscan mode

Manufactured by Zeiss

Airyscan mode is a confocal imaging technique offered by ZEISS. It utilizes a dedicated detector to improve resolution and image quality in confocal microscopy. The mode allows for high-speed, high-resolution imaging of live and fixed samples.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488 dye, by Thermo Fisher Scientific (1 mentions) Fugene hd reagent, by Promega (1 mentions) Lsm 880 confocal, by Zeiss (1 mentions) Glass bottom dish, by NEST Biotechnology (1 mentions) Ab5408, by Abcam (1 mentions) Ab5095, by Merck Group (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

2 protocols using airyscan mode

Visualizing DNMT1 and DROSHA Colocalization

Check if the same lab product or an alternative is used in the 5 most similar protocols

COS-7 or NIH/3T3 cells were grown on coverslips and co-transfected with DsRed-DNMT1 (human) (19 (link)) and HA-DROSHA (mouse) expression constructs using Fugene HD reagent (Promega) according to the manufacturer’s protocol for 48 h. Cells were then fixed with 1% paraformaldehyde. DsRed-DNMT1 was visualized by excitation with 561 nm wavelength, whilst DROSHA detection was by anti-HA antibody (2367, CST) visualized with secondary anti-mouse Alexa Fluor 488 dye (Molecular Probes). Images were acquired on a Zeiss Confocal LSM 880 using Airyscan mode (Zeiss). For quantification of DNMT1 and DROSHA co-localization, Manders split coefficients were determined using the ImageJ JACoP plugin (ImageJ.net). Coefficients represent the number of red spots (DNMT1) coinciding with green spots (DROSHA) with a threshold set at 0.4 for co-localization in individual cells.

Stathopoulou A., Chhetri J.B., Ambrose J.C., Estève P.O., Ji L., Erdjument-Bromage H., Zhang G., Neubert T.A., Pradhan S., Herrero J., Schmitz R.J, & Ooi S.K. (2017). A novel requirement for DROSHA in maintenance of mammalian CG methylation. Nucleic Acids Research, 45(16), 9398-9412.

+ Open protocol

+ Expand

Immunofluorescence and Live-Cell Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were quickly rinsed with pre-warmed DPBS, xed with pre-cooling methanol for 10 min at -20°C, and permeabilized in 0.5% Triton X-100 for 10 min. Cells were then blocked with 5% Bovine Serum Albumin for 30 min and incubated overnight at 4°C with anti-RNAPII phospho S5 (1:1500, Abcam, ab5408), anti-RNAPII phospho S2 (1:3000, Abcam, ab5095), anti-γ-Tubulin (1:1000, Sigma, T6557 For live imaging, cells in glass bottom dish (NEST, 801001) were transfected with pEGFP-Tubulin and mCherry-H2B using Lipofectamine 3000 (Invitrogen), and synchronized with double thymidine block procedure. Cells were then released into thymidine-free medium for 8 hours and imaged in PeCon environmental microscope incubator (ZEISS) at 37°C and 5% CO 2 . Images were collected on the LSM 880 confocal microscope using a 63× oil immersion objective lens with Airyscan mode (ZEISS).

Yuan K., Liu H., Yu C., Zhang N., Yang M., Can-Hua H., Hu C., Chen F., Hu X., & Zhang Z. (2021). Elevated pausing of RNA Polymerase II underlies acquired resistance to ionizing radiation.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!