1h imidazole 4 carboxylic acid

Tetraethyl orthosilicate (TEOS), 1H-imidazole-4-carboxylic acid, thionyl chloride (SOCl₂), Traut’s reagent (2-iminothiolane), Triton X-100, acetone, ethanol, glutathione (GSH), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and ammonia (30% in water) were purchased from Fisher Scientific, USA. Hexanol, cyclohexane, and (3-aminopropyl)triethoxysilane (APTES) were bought from Tokyo Chemical Industry Co., Ltd., USA. Triethylamine (TEA) and dimethyl sulfoxide (DMSO) were purchased from Alfa Aesar, USA. Bis[3-(triethoxysilyl)propyl]-disulfide (BTPD) was purchased from Gelest, Inc., USA. Methoxy-poly(ethylene glycol)-silane (mPEG-silane, M_n = 5000), amine-poly(ethylene glycol)-silane (NH₂-PEG-silane, M_n = 5000) and maleimide-poly(ethylene glycol)-silane (Mal-PEG-silane, M_n = 5000) were purchased from Biochempeg Scientific Inc., USA. All-trans-retinoic acid (ATRA) and 4-aminophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside were purchased from Santa Cruz Biotechnology, USA. Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron, USA. In vitro transcribed single guide RNAs (sgRNAs) and ssODNs were purchased from Integrated DNA Technologies, Inc., USA.

1H-imidazole-4-carboxylic acid, thionyl chloride (SOCl₂), tetraethyl orthosilicate (TEOS), tetrahydrofuran (THF), Triton X-100, acetone, ethanol, ammonia (30% in water) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were purchased from Fisher Scientific, USA. Hexanol, cyclohexane, 2-methyl-1H-imidazole (2-MIM), (3-aminopropyl)triethoxysilane (APTES), and doxorubicin hydrochloride (DOX·HCl) were bought from Tokyo Chemical Industry Co., Ltd., USA. Methoxypolyethylene glycol-N-succinimidyl ester (mPEG-NHS, M_n = 5000) and hydroxyl-polyethylene glycol-N-succinimidyl ester (HO-PEG-NHS, M_n = 5000) were obtained from Jenkem Technology, USA. Anhydrous zinc nitrate (ZnNO₃) and all-trans retinoic acid (ATRA) were purchased from Sigma–Aldrich, USA. Nuclear localization signal (NLS)-tagged streptococcus pyogenes Cas9 nuclease (sNLS–SpCas9–sNLS) was provided by Aldevron, USA.