Phase 1 of this study applied 100 ng/mL PTH 1-34 (Sigma-Aldrich, P3671), T3 (Sigma-Aldrich, T6397), or T4 (Sigma-Aldrich, T2376) during week 1 (that is, daily for days 1 to 7) or week 3 (that is, daily for days 15 to 21) of a 4-week culture period. A concentration of 100 ng/mL was chosen to maximize the potential of a cellular response [11 (link),22 (link),23 (link)]. Phase 2 evaluated the use of 25 ng/mL PTH during week 2 (that is, daily for days 8 to 14), week 3 (that is, daily for days 15 to 21), or weeks 2 to 4 (that is, daily for days 8 to 28) sequentially after 0 or 100 ng/mL T3 application during week 1. A dosing study using PTH (0, 5, 10, 25, 50, and 100 ng/mL) revealed no differences in any measured parameters, with the exception of 25 ng/mL PTH being the highest concentration to result in the highest cellular content (Additional file 1 ). A concentration of 25 ng/mL PTH was thus selected for use in Phase 2. All controls received no hormone treatment.
P3671
Manufactured by Merck Group
The P3671 is a laboratory equipment product manufactured by Merck Group. It serves as a centrifuge, a device used to separate components of a liquid mixture based on their relative density. The core function of the P3671 is to provide a controlled centrifugal force to facilitate the separation process in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using p3671
1
Hormonal Regulation of Cell Culture
2
Optimizing PTH Assay Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sigma (#P3671) for the XF24 assays, PTH was resuspended in 1 % acetic acid in H2O, aliquoted, and frozen at −80 °C.
Bachem (#4011476) For the XF96 assays, PTH was hydrated in 4 mM HCL 1 % BSA and stored as above.
Seahorse XF Plasma Membrane Permeabilizer (PMP): Either a concentration of 1.5 nM (XF24) or 1 nM (XF96) of PMP (Agilent, #102504-100) was utilized according to manufacturer instructions.
Bachem (#4011476) For the XF96 assays, PTH was hydrated in 4 mM HCL 1 % BSA and stored as above.
Seahorse XF Plasma Membrane Permeabilizer (PMP): Either a concentration of 1.5 nM (XF24) or 1 nM (XF96) of PMP (Agilent, #102504-100) was utilized according to manufacturer instructions.
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