Vab95t

Sessions were conducted in commercially available operant conditioning chambers (31 × 24 × 21 cm; ENV-008CT; Med Associates, Inc., St Albans, Vermont, USA) enclosed in ventilated, sound-attenuating cubicles (ENV-022M; Med Associates, Inc.). The side panels of the chamber were Plexiglas, and the rear and front panels were aluminum. The front panel was equipped with two response levers horizontally aligned 11.5 cm apart. Above each lever was a 2.5-cm diameter stimulus light that could be trans-illuminated white with a 100 mA bulb (lever lights). A feeder dispensed 45-mg grain-based pellets (F0165; Bio-Serv, Frenchtown, New Jersey, USA) to a 5 × 5 cm food aperture centrally located between the two levers. The rear panel was equipped with a 100 mA house light centered 2 cm from the top of the chamber. A syringe pump (PHM-100, Med Associates, Inc.) delivered i.v. infusions through Tygon tubing connected to a 22-G single channel plastic swivel (375/22PS; Instech Laboratories, Inc., Plymouth Meeting, Pennsylvania, USA) and spring tether (VAB95T; Instech Laboratories, Inc.) that attached to a vascular access button (VAB95BS; Instech Laboratories, Inc.) on the back of the rat. MED-PC IV software and a PC-compatible interface (Med Associates, Inc.) controlled experimental events and recorded data.

Sessions were conducted in commercially available operant conditioning chambers (31 × 24 × 21 cm; ENV-008CT; Med Associates, Inc., St Albans, Vermont, USA) enclosed in ventilated, sound-attenuating cubicles (ENV-022M; Med Associates, Inc.). The side panels of the chamber were Plexiglas, and the rear and front panels were aluminum. The front panel was equipped with two response levers horizontally aligned 11.5 cm apart. Above each lever was a 2.5-cm diameter stimulus light that could be transilluminated white with a 100 mA bulb (lever lights). A feeder dispensed 45-mg grain-based pellets (F0165; Bio-Serv, Frenchtown, New Jersey, USA) to a 5 × 5 cm food aperture centrally located between the two levers. The rear panel was equipped with a 100 mA house light centered 2 cm from the top of the chamber. A syringe pump (PHM-100, Med Associates, Inc.) delivered i.v. infusions through Tygon tubing connected to a 22-G single channel plastic swivel (375/22PS; Instech Laboratories, Inc., Plymouth Meeting, Pennsylvania, USA) and spring tether (VAB95T; Instech Laboratories, Inc.) that attached to a vascular access button (VAB95BS; Instech Laboratories, Inc.) on the back of the rat. MED-PC IV software and a PC-compatible interface (Med Associates, Inc.) controlled experimental events and recorded data.