5 6 carboxyfluorescein succinylester

M. bovis BCG (DSMZ No. DSM 43990) was grown as described previously57 (link). For microscopy, BCG was fluorescently labelled with 5-(6)-carboxyfluorescein-succinylester (Sigma Aldrich) as described previously58 (link).
Human acute monocytic leukaemia cell line THP-1 (DSMZ No. ACC 16), human cervix carcinoma cell line HeLa (ATCC No. CCL-2), human monocytic cell line U937 (DSMZ No. ACC 5), human type II pneumocyte cell line A549.1 (ATCC No. CCL 185), human colorectal adenocarcinoma cells HT-29 (DSMZ No.: ACC 299) and its sub clone HT-29/B659 (link) were cultured as described previously5 (link)57 (link)60 (link)61 (link) or following the protocols for nucleofection (Lonza). Total RNA from cell lines was isolated and quality controlled as described earlier62 (link).

M. bovis BCG (DSMZ No. DSM 43990) was grown as previously described (Sharbati-Tehrani et al., 2004 (link)). For microscopy, BCG was fluorescently labeled with 5-(6)-carboxyfluorescein-succinylester (Sigma Aldrich) following an earlier described protocol (Agerer et al., 2004 (link)). The human acute monocytic leukemia cell line THP-1 (DSMZ ACC 16) was cultured in suspension using RPMI 1640 (Biochrom AG) supplemented with 10% fetal bovine serum superior (Biochrom AG) and gentamycin (zur Bruegge et al., 2014 (link)). Infection experiments were performed as described previously (Pawar et al., 2016 (link)).