F e2m 48

Manufactured by Natus

Sourced in United States

The F-E2M-48 is a laboratory equipment product designed for scientific and research applications. It is a multi-channel electrophysiological signal recording and analysis system. The device is capable of acquiring and processing various physiological signals, such as electroencephalography (EEG), electromyography (EMG), and electrocardiography (ECG).

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Lab products found in correlation

Model 701 a, by Aurora Scientific (1 mentions) Model 305c, by Aurora Scientific (1 mentions)

3 protocols using f e2m 48

Muscle Force Measurement in Mice

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Mice were anesthetized under isoflurane and placed on an aluminum platform maintained at 38°C. The knee was securely fastened to an immobile horizontal support. The foot was taped to the foot pad of a calibrated dual mode muscle lever system (model 305C-LR, Aurora Scientific). Platinum needle electrodes (model F-E2M-48, Grass Technologies) were inserted superficially onto the TA muscle for muscle stimulation via a biphasic muscle stimulator (model 701 A, Aurora Scientific). Twitch force was determined with a single stimulus consisting of a 200-µs square-wave pulse. The tetanic force-frequency relationship was generated with square-wave stimuli of 200 ms.

Pearsall R.S., Davies M.V., Cannell M., Li J., Widrick J., Mulivor A.W., Wallner S., Troy M.E., Spaits M., Liharska K., Sako D., Castonguay R., Keates S., Grinberg A.V., Suragani R.N, & Kumar R. (2019). Follistatin-based ligand trap ACE-083 induces localized hypertrophy of skeletal muscle with functional improvement in models of neuromuscular disease. Scientific Reports, 9, 11392.

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Electrochemical Reshaping of Cartilage Tissue

Cited 1 time

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After 15 minutes of immersion in PBS, the 2 mm thick section was curved using EMR to create the superior portion of the 2-part helical rim. EMR is a non-thermal reshaping technology that creates in situ redox changes in tissue leading to local stress relaxation and is described in detail in the literature.^{19 (link)–29} The reshaping process was performed by first securing the 2 mm cartilage specimen to a cylindrical cork mandrel (diameter=15 cm) using needles. The mandrel provided a degree of overcorrection to compensate for shape memory effects (Figure 1E–G). Thereafter, platinum coated anode and cathode electrodes (F-E2M-48, Grass Technologies, West Warwick, RI) were spaced evenly 2 mm apart and inserted into the specimen spanning the entire circumference of the mandrel. The electrodes were connected to terminals of a DC power supply (Model PPS-2322, Amrel, Arcadia, CA, USA), and dosimetry of 5 V for 3 minutes was applied.^{20 (link)–30 (link)} The electrodes were then removed, and the tissue and mandrel (with needles in place) were placed in PBS for 15 minutes to allow for rehydration and stabilization of shape. No other cartilage segments were reshaped using EMR.

Gandy J.R., Lemieux B., Foulad A, & Wong B.J. (2016). Conserving Cartilage In Microtia Repair: The Modular Component Assembly Approach To Rebuilding A Human Ear. JAMA facial plastic surgery, 18(2), 120-127.

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In Situ Muscle Force Measurement

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In situ muscle force measurements were performed, as previously described [12 (link)]. Briefly, a nonsurvival surgery was performed to expose the left gastrocnemius muscle and calcaneal tendon, the latter of which was cut and attached via silk suture to the force lever arm and the leg stabilized by inserting a disposable monopolar needle (902-DMF37-S, Natus Neurology, WI, USA) through the knee joint. Twitch and tetanic force were recorded following stimulation of the sciatic nerve with 200 ms square pulses via insulated monopolar needles (F-E2M-48, Grass Technologies, RI, USA). A high-speed servo motor-based apparatus (Model 305C, Aurora Scientific, ON, Canada) was used to measure force output. Stimulation current and resting tension were adjusted to maximize twitch force produced by a single stimulus pulse. All subsequent isometric tetanic force data were collected at this stimulation current and resting tension. Isometric force frequency relationship was recorded after stimulation by a train of square wave stimuli and maximum isometric tetanic force was recorded. Animals were then euthanized.

Nagy J.A., Kapur K., Taylor R.S., Sanchez B, & Rutkove S.B. (2018). Electrical impedance myography as a biomarker of myostatin inhibition with ActRIIB-mFc: a study in wild-type mice. Future Science OA, 4(6), FSO308.

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