The RNA from the Ae. albopictus midguts were extracted by TRIzol reagent (AG RNAex Pro RNA, Accurate Biology, Hunan, China) according to the manufacturer’s instructions and reverse transcribed to cDNA using a GoScript Reverse Transcriptase System (Promega, Madison, WI, United States). A Power Green qPCR Mix kit (SYBR Green 1) (GDSBio, Guangzhou, China) was used to quantify the cDNA to detect the viral load and related gene expression in the midgut of Ae. albopictus. Rps7 (encoding ribosomal protein S7) was used as an internal reference gene. The primers used are shown in Supplementary Table 1 .
Ag rnaex pro rna
Manufactured by Accurate Biology
Sourced in China
AG RNAex Pro RNA is a laboratory equipment designed for the extraction and purification of RNA from various biological samples. It functions as a high-performance tool for isolating RNA from cells, tissues, and other sources, enabling researchers to obtain pure and high-quality RNA for further analysis and experimentation.
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Lab products found in correlation
2 protocols using ag rnaex pro rna
1
Viral Load and Gene Expression Analysis in Ae. albopictus Midgut
2
RNA Extraction and qPCR Analysis
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Total RNA was extracted from cells and spleen tissues using AG RNA ex Pro RNA (AG21101, Accurate Biology). Potential genomic DNA contaminants of RNA samples were eliminated by digesting with DNase I before the reverse transcription step.
Total RNA samples were reverse transcribed for complementary DNA (cDNA) with the Evo M-MLV Mix Kit with gDNA Clean for qPCR(AG11728, Accurate Biology). Real time PCR was performed using an SYBR Green Premix Pro Taq HS qPCR kit (AG11701, Accurate Biology) on a LightCycler 96 (Real-Time PCR Detection System, Roche). The data were normalized to the endogenous control GAPDH, and the fold change was calculated using relative quanti cation (2^-∆∆Ct).
Total RNA samples were reverse transcribed for complementary DNA (cDNA) with the Evo M-MLV Mix Kit with gDNA Clean for qPCR(AG11728, Accurate Biology). Real time PCR was performed using an SYBR Green Premix Pro Taq HS qPCR kit (AG11701, Accurate Biology) on a LightCycler 96 (Real-Time PCR Detection System, Roche). The data were normalized to the endogenous control GAPDH, and the fold change was calculated using relative quanti cation (2^-∆∆Ct).
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