Vegf a121

Manufactured by PromoCell

Sourced in United States

VEGF-A121 is a recombinant human vascular endothelial growth factor A isoform. It is a protein involved in the regulation of angiogenesis and vasculogenesis.

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Lab products found in correlation

3 protocols using vegf a121

VEGFR2 and NFAT Signaling in Endothelial Cells

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Antibodies: goat-anti-VEGFR2 (R&D Systems, Minneapolis, MN, USA), goat-anti-VEGFR1 (R&D Systems), rabbit-anti-ERK1/2, mouse-anti-phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), rabbit-anti-p38, rabbit-anti-phospho-p38 (Thr¹⁸⁰/Tyr¹⁸²), rabbit-anti-phospho-VEGFR2 (Tyr¹¹⁷⁵), rabbit-anti-eNOS, rabbit-anti-phospho-eNOS(Ser¹¹⁷⁷), rabbit-anti-phospho-PLCγ1(Tyr⁷⁸³), rabbit-anti-NFATc2 (Cell Signaling Technology, Danvers, MA, USA), mouse anti-PECAM-1 (CD31), rabbit-anti-PLCγ1 (Santa Cruz Biotechnology, Dallas, TX, USA) and mouse-anti-α-tubulin (Sigma-Aldrich, Poole, UK). Reagents: Endothelial cell growth medium (ECGM) was from PromoCell (Heidelberg, Germany). Scrambled and NFATc2 siRNA duplexes were purchased as siGENOME SMARTpools from Dharmacon (Thermo Scientific, Lafayette, USA). Recombinant human VEGF-A₁₆₅ was from Genentech Inc. (San Francisco, CA, USA) and VEGF-A₁₂₁ was from Promocell.

Fearnley G.W., Bruns A.F., Wheatcroft S.B, & Ponnambalam S. (2015). VEGF-A isoform-specific regulation of calcium ion flux, transcriptional activation and endothelial cell migration. Biology Open, 4(6), 731-742.

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Endothelial Cell VEGF Signaling Pathway

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Antibodies were goat anti-VEGFR2 (R&D Systems, Minneapolis, MN), rabbit anti-ERK1/2, mouse anti–phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴), rabbit anti-p38, rabbit anti–phospho-p38 (Thr¹⁸⁰/Tyr¹⁸²), rabbit anti–phospho-VEGFR2 (Tyr¹¹⁷⁵), rabbit anti–ATF-2, rabbit anti–phospho-ATF-2 (Thr⁷¹), rabbit anti-NRP1 (Cell Signaling Technology, Danvers, MA), mouse anti–α-tubulin, mouse anti–PECAM-1 (CD31; Santa Cruz Biotechnology, Santa Cruz, CA), and mouse anti–VCAM-1 (DAKO, Glostrup, Denmark).
Reagents were as follows. Endothelial cell growth medium (ECGM) was from PromoCell (Heidelberg, Germany). Scrambled, ATF-2, NRP1, and VCAM1 siRNA duplexes were purchased as siGENOME SMARTpools from Dharmacon (Thermo Scientific, Lafayette, CO) unless otherwise stated in the figure legends. Recombinant human VEGF-A₁₆₅ was from Genentech (San Francisco, CA), and VEGF-A₁₂₁ was from Promocell.

Fearnley G.W., Odell A.F., Latham A.M., Mughal N.A., Bruns A.F., Burgoyne N.J., Homer-Vanniasinkam S., Zachary I.C., Hollstein M.C., Wheatcroft S.B, & Ponnambalam S. (2014). VEGF-A isoforms differentially regulate ATF-2–dependent VCAM-1 gene expression and endothelial–leukocyte interactions. Molecular Biology of the Cell, 25(16), 2509-2521.

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VEGFR Signaling Pathway Analysis

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Antibodies: goat-anti-VEGFR1 (#AF321), goat-anti-VEGFR2 (#AF357), rabbit-anti-phospho-VEGFR2-Y1214 (#AF1766) (R&D Systems, Minneapolis, MN, USA), rabbit-anti-Akt (#9272S), rabbit-anti-phospho-Akt (S473; #4060B), rabbit-anti-ERK1/2 (#9102S), mouse-anti-phospho-ERK1/2 (T202/Y204; #9106S), rabbit-anti-neuropilin 1 (NRP1; #3725S), rabbit-anti-phospho-VEGFR2-Y951 (#4991S), rabbit-anti-phospho-VEGFR2-Y1059 (#3817S), rabbit-anti-phospho-VEGFR2-Y1175 (#2478S; Cell Signaling Technology, Danvers, MA, USA), rabbit-anti-phospho-VEGFR2-Y1054 (Clone D1W; #04-894; Merck Millipore, Watford, UK), mouse-anti-α-tubulin (Clone DM1A; #T6199; Sigma Aldrich, Poole, UK), mouse-anti-PECAM-1 (CD31; #sc-65260; Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-ubiquitin (FK2; #14220; Caymen Chemical, MI, USA), mouse-anti-clathrin heavy chain antibody (X22; #ab2731; Abcam, Cambridge, UK), sheep-anti-TGN46 (#AHP500GT; AbD Serotec, Oxford, UK). Endothelial cell growth medium (ECGM) was from PromoCell (Heidelberg, Germany). Recombinant human VEGF-A₁₆₅ was from Genentech Inc. (San Francisco, CA, USA), both VEGF-A₁₂₁ and VEGF-A₁₄₅ was from Promocell.

Fearnley G.W., Smith G.A., Abdul-Zani I., Yuldasheva N., Mughal N.A., Homer-Vanniasinkam S., Kearney M.T., Zachary I.C., Tomlinson D.C., Harrison M.A., Wheatcroft S.B, & Ponnambalam S. (2016). VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biology Open, 5(5), 571-583.

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