Sticky slide vi0.4 flow chamber

To generate RNA tracks, SH-PEG barriers were initially patterned onto the thin gold film using established protocols.³⁴ Briefly, 30 μL of a 1 mM SH-PEG solution in ethanol was applied to a PDMS stamp with 3 μm wide parallel lines spaced at a 3 μm pitch. Next, the SH-PEG solution was dried under a stream of N₂ for 1 min and the remaining solution was wicked away. The coated stamp was then brought into contact with the gold film surface for 30 sec. After contact, the stamp was peeled away from the substrate and used to print repeatedly without re-applying additional SH-PEG ink. After patterning the SH-PEG barriers onto the surface, the IBIDI sticky-Slide VI^0.4 flow chamber was adhered to the gold slide, and the substrate was prepared as described above in the “Fabrication of RNA monolayers” section. Note that the RNA track preparation protocol used a 6 hr incubation with the DNA anchor solution rather than 12 hrs.