Oleogels were analyzed at room temperature with a polarized light microscope (Olympus System Microscope model BX51TF, Olympus America Inc., Center Valley, PA, USA) equipped with a digital camera (Olympus EX300, Olympus America Inc., Center Valley, PA, USA). Pictures were taken at a magnification of 50× and 200×.
Ex300
Manufactured by Olympus
Sourced in United States
The Olympus EX300 is a compact and portable laboratory equipment designed for various scientific applications. It features a high-resolution imaging system and user-friendly controls for easy operation. The core function of the EX300 is to provide reliable and accurate data collection for researchers and scientists.
Automatically generated - may contain errors
5 protocols using ex300
1
Oleogel Microscopic Analysis
2
Microscopic Analysis of Sterol-Based Oleogels
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Sterol-based oleogels were cut and placed in a cover slip and analysed at room temperature with a polarized light microscope (Olympus System Microscope model BX51TF, Olympus America Inc., Center Valley, PA, USA) equipped with a digital camera (Olympus EX300, Olympus America Inc.). Pictures were taken at a magnification of 120× and 320×. Images were segmented using the threshold colour plugin available in ImageJ software. This plugin enables an image segmentation in distinctive black and white colour areas defined using an automatically calculated threshold. 21
3
Organogel Micrograph Imaging
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Micrographs of organogels (4 %w/w) were obtained under polarized light microscope Olympus System Microscope model BX 51 (Olympus America Inc. Center Valley, PA, USA) equipped with a digital camera model Olympus EX300 (Olympus America Inc. Center Valley, PA, USA).
Samples were melted at 80 °C and a small drop put over a heated glass slide and covered with a glass coverslip. The glass slides were crystallized on at 5 and 25°C for 24 hours and analyzed at 25°C. Samples were treated using Image cell Sens Standard 1.7.1.
(Olympus, USA).
Samples were melted at 80 °C and a small drop put over a heated glass slide and covered with a glass coverslip. The glass slides were crystallized on at 5 and 25°C for 24 hours and analyzed at 25°C. Samples were treated using Image cell Sens Standard 1.7.1.
(Olympus, USA).
4
Polarized Light Microscopy of Organogels
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Micrographs of organogels were obtained under a polarized light microscope (Olympus System Microscope model BX51TF, Olympus America Inc., Center Valley, PA, USA) equipped with a digital camera (Olympus EX300, Olympus America Inc., Center Valley, PA, USA). The samples after preparation at 80 °C were poured directly in the support and conditioned at 25 °C during 24 h before being analysed at room temperature. Pictures were taken at a magnification of 80×.
5
Organogel Morphology Characterization
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The organogels were placed on a glass slide and covered with a coverslip. Then, organogels were conditioned at 20 °C for 24 h and examined under constant temperature. The morphologies of the crystals of SBO/PGE and SBO/PPGE organogels (4% w/w) were viewed under a polarized light microscope (Olympus System Microscope model BX 50, Olympus America Inc., Center Valley, PA, USA) equipped with the digital camera Olympus EX300 (Olympus America Inc., Center Valley, PA, USA). Photographs were taken in different fields and visuals, and the resulting images were evaluated using the software Image Pro-Plus 7.0.1 for Windows by Media Cybernetics (Bethesda, MD, USA) with a magnification of 4x (Rocha et al., 2013) (link).
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