Fluorescein‐conjugated dextran (D3306, molecular weight: 3 kDa, Dex‐3) was purchased from Invitrogen. Gd‐DPTA (molecular weight: 938 Da) was obtained from CONSUN. HiLyte Fluor‐488‐labeled recombinant human α‐syn (AS‐55457, molecular weight: 14 kDa HiLyte‐488‐α‐syn) was purchased from Anaspec. Adeno‐associated virus expressing A53T‐α‐syn (AAV‐A53T) was designed by OBio Technology. Primary antibody anti‐AQP4 (rabbit, AB3594), anti‐α‐syn (mouse, 36–008; Syn211), and anti‐tyrosine hydroxylase (rabbit, AB152; anti‐TH) were purchased from Millipore. Anti‐PDGF‐B (rabbit, DF6328) was purchased from Affinity Biosciences, and anti‐PDGFRb (mouse, sc‐374573) was purchased from Santa Cruz Biotechnology. β‐actin (mouse, ab008‐100), horseradish peroxidase‐conjugated anti‐rabbit and anti‐mouse IgG (70‐gam007, 70‐gar007) were purchased from Multisciences. Secondary antibodies, including Cy3‐conjugated donkey anti‐rabbit and Cy5‐conjugated donkey anti‐rabbit, were purchased from Jackson ImmunoResearch. DNaseI and 4′,6‐diamidine‐2′‐phenylindole dihydrochloride (DAPI) were purchased from Sigma‐Aldrich. RNAiso Plus (9109), PrimeScrip RT Master Mix (RR036A) and SYBR Premix Ex Taq II (RR820A) were purchased from TaKaRa.
Cy5 conjugated donkey anti rabbit
Manufactured by Jackson ImmunoResearch
Sourced in United Kingdom
Cy5-conjugated donkey anti-rabbit is a secondary antibody used for detection and visualization in various immunological techniques. It is conjugated with the fluorescent dye Cy5, which allows for sensitive detection of target proteins. The antibody is produced in donkeys and specifically binds to rabbit primary antibodies, enabling the identification and localization of rabbit-recognized antigens.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidine 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
Primescrip rt master mix, by Takara Bio (1 mentions)
Ab3594, by Merck Group (1 mentions)
Rnaiso plus 9109, by Takara Bio (1 mentions)
Anti pdgfrb, by Santa Cruz Biotechnology (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Cy3 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions)
β actin, by MultiSciences Biotech (1 mentions)
Alexa 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Cy3 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Anti phospho src tyr416, by Cell Signaling Technology (1 mentions)
Anti α catenin, by Merck Group (1 mentions)
Anti β catenin, by BD (1 mentions)
Anti e cadherin, by BD (1 mentions)
Goat serum, by Thermo Fisher Scientific (1 mentions)
Cy3 conjugated goat anti mouse, by Jackson ImmunoResearch (1 mentions)
Anti ki67, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Merck Group (1 mentions)
3 protocols using cy5 conjugated donkey anti rabbit
1
Fluorescent Markers for Neurodegeneration
2
Immunofluorescence staining and RhoA biosensors
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The following antibodies were used for immunofluorescence staining: anti-E-cadherin (1:250; BD Biosciences), anti-β-catenin (1:250; BD Biosciences), anti-α-catenin (1:250; Sigma Aldrich), anti-Phospho-Src Tyr416 (1:50; Cell Signaling), Cy5 conjugated donkey anti-rabbit (1:800; Jackson Laboratory), and Cy3 conjugated goat anti-mouse (1:1000; Invitrogen). In addition, Alexa488 conjugated phalloidin (1:40; Invitrogen) was used to stain actin filaments. pRBD-YPet was constructed by inserting the cDNA sequence encoding aa 7–89 of mouse rhotekin into the EcoRI/BamHI sites of pYPet-N1. The YPet sequence was amplified from pCEP4YPet-MAMM (Addgene plasmid 14032) and cloned into AgeI/NotI sites of pEYFP-N1 (Clontech), generating the pYPet-N1 (Nguyen and Daugherty 2005 (link)). The pECFP-RhoA construct was previously described (Picard et al. 2009 (link)).
3
Immunofluorescent Characterization of Cell Cultures
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Cultures were fixed under proliferation conditions or after 10 days of differentiation with 4% buffered paraformaldehyde (PFA; Sigma-Aldrich). Cells on coverslips were incubated overnight at 4°C in 1× phosphate-buffered saline (PBS) containing 1% goat serum (Gibco; Thermo Fisher Scientific) and 0.25% Triton X-100 (Sigma-Aldrich) with the primary monoclonal antibodies anti-Ki-67 (1:200; Thermo Fisher Scientific) or anti-b-III-tubulin (1:2,000; Sigma-Aldrich) and primary polyclonal antibody against TH (1:1,000; Chemicon, Merck Chemicals and Life Science GesmbH, Madrid, Spain). Samples were washed and incubated afterward for 1 h with appropriate fluorescent secondary antibodies: Cy5-conjugated donkey antirabbit (1:200; Jackson ImmunoResearch, Suffolk, UK), Cy3-conjugated goat anti-mouse (1:200; Jackson Immuno Research), and counterstained with Hoechst 33342 (1:400; Life Technologies, Thermo Fisher Scientific) to label nuclei.
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