Atcc crl 1573

Chicken ovalbumin (OVA)-transfected EL-4 (EG7) cell line derived from C57BL/6J mice and human embryonic kidney cell line 293 (HEK293, ATCC® CRL-1573™, LGC Standards S.r.l., Milano, Italy), THP-1 cell line (ATCC® TIB-202™, LGC Standards S.r.l., Milano, Italy), THP1-Blue™ NF-κB cell line (InvivoGen, CA, USA), MCA203 cell line, a fibrosarcoma induced by 3-methylcholanthrene (a kind gift by Prof. M.P.Colombo) and MCA205 cell line, a mouse sarcoma (a kind gift by Prof. L. Zitvogel) were grown in DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 2 mM _L-glutamine (Euroclone, Milano, Italy), 10 mM HEPES (Euroclone, Milano, Italy), 20 µM β-mercaptoethanol (Sigma-Aldrich, Saint Louis, MO, USA), 150 U/ml streptomycin (Euroclone, Milano, Italy), 200 U/ml penicillin (Euroclone, Milano, Italy) and 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA). MSC2 is an immortalized cell line derived from Balb/c mice^{26 (link)} and it was cultured in RPMI 1640 (Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM _L-glutamine (Euroclone, Milano, Italy), 10 mM HEPES (Euroclone, Milano, Italy), 150 U/ml streptomycin (Euroclone, Milano, Italy), 200 U/ml penicillin (Euroclone, Milano, Italy) and 10% heat-inactivated FBS (Superior, Merck, Darmstadt, Germany). All cell lines were tested to be free from Mycoplasma contamination by PCR screening.

Human Embryonic Kidney 293 (HEK) cells (ATCC-CRL-1573, LGC Standards GmbH, Wesel, Germany), HeLa cells (ATCC-CRM-CCL-2, LGC Standards GmbH, Wesel, Germany) and human-derived fibroblasts (control lines 3/2 and 3/3, C9orf72 ALS patient lines 24/4 and 24/5) were grown and maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) with high glucose and L-Glutamine (Gibco, Waltham, US) supplemented with 10% fetal bovine serum (FBS, Gibco), 5% non-essential amino acids (NEAA, Gibco) and penicillin/streptomycin (100 µg/ml). For transfection purposes, antibiotics were not added to media. Primary human fibroblasts were obtained from skin biopsies of ALS patients and controls with the approval of the Ethical Committee of the University Hospitals Leuven (S50354). Clinical information is indicated in Supplementary table 2 (online resource). Human C9orf72 ALS patient iPSC lines and their isogenic controls were obtained and were published before [61 (link)]. Maintenance of iPSCs and motor neuron differentiation were performed as previously described [24 (link)].