Single-molecule imaging experiments were conducted on a custom-built Nikon Ti microscope. The microscope is equipped with a ×100 Oil-immersion objective lens (N.A. = 1.49), a multi-band dichroic (405/488/561/633 BrightLine quad-band bandpass filter, Semrock, USA) and a piezo z-stage (ASI, USA), a filter wheel (Sutter Instrument, USA) and a stage top incubator (Tokai Hit, Japan). The lasers were focused into the back pupil plane of the objective to generate wide-field illumination. A Nikon N-STORM module was used steer the incidence angle of the laser for generating inclined illumination. The emission was collected by the same objective passing through an emission filter (617/73, Semrock) in front of sCMOS camera (Prime 95B, Teledyne Photometrics). The microscope, lasers and the camera were controlled through NIS-Elements (Nikon, USA).
561 633 brightline quad band bandpass filter
Manufactured by IDEX Corporation
Sourced in United States
The 405/488/561/633 BrightLine quad-band bandpass filter is a multi-band optical filter designed to transmit specific wavelengths of light. It is capable of isolating four distinct wavelengths: 405 nm, 488 nm, 561 nm, and 633 nm. The filter provides high transmission efficiency and excellent out-of-band blocking, making it suitable for use in various laboratory and research applications.
Automatically generated - may contain errors
3 protocols using 561 633 brightline quad band bandpass filter
1
Single-Molecule Imaging Microscopy Setup
2
Single-Molecule Imaging Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-molecule
imaging experiments were conducted on a custom-built Nikon Ti microscope
coupled with a 100× oil-immersion objective lens (NA = 1.49),
a multiband dichroic (405/488/561/633 BrightLine quad-band bandpass
filter, Semrock, USA), and a piezo z-stage (ASI, USA). The lasers
were focused into the back pupil plane of the objective to generate
wide-field illumination. A Nikon N-STORM module was used to control
the angle of the laser beam for generating inclined illumination.
The emission was collected by the same objective passing through a
quadband bandpass emission filter (FF01-446/523/600/677-25, Semrock,
USA) in front of an sCMOS camera (Prime 95B, Teledyne Photometrics).
The microscope, lasers, and the camera were controlled through NIS-Elements
(Nikon, USA). A 488 nm laser was used excite the QDs.
imaging experiments were conducted on a custom-built Nikon Ti microscope
coupled with a 100× oil-immersion objective lens (NA = 1.49),
a multiband dichroic (405/488/561/633 BrightLine quad-band bandpass
filter, Semrock, USA), and a piezo z-stage (ASI, USA). The lasers
were focused into the back pupil plane of the objective to generate
wide-field illumination. A Nikon N-STORM module was used to control
the angle of the laser beam for generating inclined illumination.
The emission was collected by the same objective passing through a
quadband bandpass emission filter (FF01-446/523/600/677-25, Semrock,
USA) in front of an sCMOS camera (Prime 95B, Teledyne Photometrics).
The microscope, lasers, and the camera were controlled through NIS-Elements
(Nikon, USA). A 488 nm laser was used excite the QDs.
3
Single-Molecule Imaging Microscopy Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single-molecule imaging experiments were conducted on a custom-built Nikon Ti microscope. The microscope is equipped with a 100 × oil-immersion objective lens (N.A. = 1.49), a multi-band dichroic (405/488/561/633 BrightLine quad-band bandpass filter, Semrock, USA) and a piezo Z-stage (ASI, USA), a filter wheel (Sutter Instrument, USA) and a stage top incubator (Tokai Hit, Japan). The lasers were focused on the back pupil plane of the objective to generate wide-field illumination. Nikon N-STORM module was used to steer the incidence angle of the laser for generating inclined illumination. The emission was collected by the same objective passing through an emission filter (617/73, Semrock) in front of the sCMOS camera (Prime 95B, Teledyne Photometrics). The microscope, lasers, and the camera were controlled through NIS-Elements (Nikon, USA).
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