Inhibitors were added to cells prior to imaging at the following concentrations and timepoints: Cdc42 inhibitor ZCL 278 (Cayman Chemical, 14849), 7.5 μM, 2 hours prior to imaging; Rac1 GEF inhibitor NSC 23766 (Cayman Chemical, 13196), 50 μM, 24 hours prior to imaging; Arp2/3 inhibitor CK-666 (Millipore-Sigma, SML0006), 500 μM, 6 hours prior to imaging; actin polymerization inhibitor cytochalasin D (Cayman Chemical, 11330), 10 μM, 1 hour prior to imaging.
Zcl 278
Manufactured by Cayman Chemical
ZCL 278 is a chemical compound used in laboratory research. It functions as a signaling pathway inhibitor.
Automatically generated - may contain errors
2 protocols using zcl 278
1
Inhibiting Cytoskeletal Dynamics in Cells
2
Inhibiting p22 Downstream Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To perturb downstream signaling of p22, 8-9 hours post transfection, Latrunculin A (abcam), Wiskostatin (Sigma Aldrich), CK666 (Sigma Aldrich), smifH2 (CalBioChem), ZCL278 (Cayman Chemical), ML141 (Cayman Chemical) were added to complete media at specified concentrations. DMSO was used as vehicle control, and 36 hours post transfection the cells were imaged to quantify the extent of cell-cell fusion.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!