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6 galactobiose

Manufactured by Merck Group
Sourced in Germany

6-galactobiose is a disaccharide carbohydrate composed of two galactose molecules. It is a key component in various biological processes and can be used in laboratory applications. The core function of 6-galactobiose is to serve as a substrate for specific enzymatic reactions and as a structural building block in certain glycan molecules.

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2 protocols using 6 galactobiose

1

Enzymatic Conversion of Lactose

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Aspergillus oryzae β-galactosidase (E. C. 3.2.1.23), lactulose, d-fructose, 6-galactobiose, and 50% glutaraldehyde were purchased from Sigma-Aldrich (Steinheim, Germany). Sepharose 4B was supplied by Pharmacia Biotechnology (Uppsala, Sweden). d-galactose, raffinose and o-nitrophenyl-β-d-galactopyranoside (ONPG) from Fluka (Steimheim, Germany). Fructooligosaccharides (Orafti P95) and inulin (Orafti HP) were kindly gifted by Orafti (Tienen, Belgium).
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2

Characterization of Carbohydrate Sources

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Lactose was obtained from Scharlau (Barcelona, Spain). D-GaLactose, Dglucose, D-fructose, lactulose, raffinose, 6-galactobiose, phenyl-β-D-glucoside and onitrophenyl β-D-galactopyranoside (oNPG) were purchased from Sigma-Aldrich Co.
(Steinheim, Germany). D-Glucose and Lactose for yeast culture media were obtained from Panreac (Barcelona, Spain), bacteriological peptone was acquired from Cultimed (Barcelona, Spain) and yeast extract and agar were purchased from Pronadisa (Madrid, Spain). Ultrapure water (18.2 M Ω-cm, with levels of 1-5 ng/mL total organic carbon and <0.001 EU/mL pyrogen) produced in-house with a laboratory water purification system (Milli-Q Synthesis A10, Millipore, Billerica, MA, USA) was used throughout.
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