Anti cd11b ab

Immunofluorescence assay was performed using the paraffin sections of the hippocampus. Following deparaffinization and rehydration, the sections were incubated in citrate buffer and then blocked with 3% bovine serum albumin for 30 min. Subsequently, the specimens were incubated with the primary antibodies (anti-Iba1 ab diluted at 1 : 500, anti-CD11b ab and anti-IL-17R diluted at 1 : 100, Abcam, Cambridge, UK) at 4°C overnight and then labeled with the secondary antibodies (CY3-conjugated goat anti-rabbit IgG or FITC-conjugated goat anti-rat IgG, Sigma-Aldrich, St. Louis, MO, USA) diluted at 1 : 400 at 37°C for 30 min. The nucleus was stained with DAPI (Sigma-Aldrich, St. Louis, MO, USA). The sections were dehydrated and mounted for detection by using an inverted fluorescence microscope (Leica, Wetzlar, Germany).

β-PAE was obtained from patchouli oil with a purity over 98% as described [12 (link)]. Anti-Nrf2 ab (clone103, MABE1799), anti-HO-1 ab (cloneHO-1-1, 374087), anti-Histone H3 ab(H0164), anti-GAPDH ab(G9545), and ML385 were purchased from Sigma-Aldrich (St.Louis, MO, US). Anti-iNOS ab(D6B6S), anti-Sirt1 ab and the secondary antibodies (Goat anti-rat CY3-conjugated IgG, Goat anti-rabbit FITC-conjugated IgG) were purchased from Cell Signaling Technology (MA, USA). Anti-CD11b ab and anti-Iba-1 ab were obtained from Abcam (Cambridge, UK).
β-PAE or ML385 was prepared in 3μL according to the designated dose. Mice were i.c.v injected with β-PAE immediately following CLP, and then treated with or without ML385 at 2 hours post procedures. For the control purpose, mice in control groups received equal volume of saline. A stereotactic instrument was used to ensure the accuracy and reliability.