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Il 6 enzyme linked immunosorbent assay elisa kit

Manufactured by Fortis Life Sciences
Sourced in United States

The IL-6 enzyme-linked immunosorbent assay (ELISA) Kit is a laboratory instrument used to quantitatively measure the levels of interleukin-6 (IL-6) in various biological samples, such as cell culture supernatants, serum, and plasma. It employs the ELISA technique to detect and quantify the presence of the IL-6 protein.

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4 protocols using il 6 enzyme linked immunosorbent assay elisa kit

1

Cytokine and Apoptosis Signaling Assay

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d-Pinitol (purity 95%) was purchased from Sigma-Aldrich Co. (St Louis,
MO, USA). Rats-specific TNF-α, IL-1β, and IL-6 enzyme-linked immunosorbent assay
(ELISA) Kit were obtained from Bethyl Laboratories Inc. (Montgomery, TX, USA).
The primary antibodies of caspase-3, caspase-9, caspase-12, AFT4 (Activating
transcription factor-4), AFT6α (Activating transcription factor-6α), XBP-1
(X-box binding protein 1), ERK-1/2 (Extracellular signal-regulated kinase-1/2),
and p38 were purchased from Abcam (Cambridge, MA, USA).
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2

Cytokine and Inflammatory Mediator Analysis

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Rat speci c TNF-α, IL-1β, and IL-6 enzyme-linked immunosorbent assay (ELISA) Kit were obtained from Bethyl Laboratories Inc., Montgomery, TX, USA. The primary antibodies of NF-kβ, Ikβα, COX-2, P2X7, and GAPDH were purchased from Abcam, Cambridge, MA, USA. Microliter syringe (Hamilton, Bonaduz, Switzerland) was obtained from Anchrome Enterprises (I) Pvt. Ltd (Mumbai, India). Methanol, ethyl acetate, toluene, formic acid, precoated thin layer chromatographic (TLC) silica gel plates (Kieselgel 60, F-254, 0.2 mm) was purchased from Merck Life Science Pvt Ltd, India. High-performance thin-layer chromatography (HPTLC) spectra were recorded on a Linomat V (Camag, Muttenz, Switzerland).
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3

Cytokine and Inflammatory Mediator Analysis

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Rat speci c TNF-α, IL-1β, and IL-6 enzyme-linked immunosorbent assay (ELISA) Kit were obtained from Bethyl Laboratories Inc., Montgomery, TX, USA. The primary antibodies of NF-kβ, Ikβα, COX-2, P2X7, and GAPDH were purchased from Abcam, Cambridge, MA, USA. Microliter syringe (Hamilton, Bonaduz, Switzerland) was obtained from Anchrome Enterprises (I) Pvt. Ltd (Mumbai, India). Methanol, ethyl acetate, toluene, formic acid, precoated thin layer chromatographic (TLC) silica gel plates (Kieselgel 60, F-254, 0.2 mm) was purchased from Merck Life Science Pvt Ltd, India. High-performance thin-layer chromatography (HPTLC) spectra were recorded on a Linomat V (Camag, Muttenz, Switzerland).
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4

D-pinitol Suppresses Inflammatory Responses

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D-pinitol (purity 95%) was purchased from Sigma-Aldrich Co., St Louis, MO, USA. Rats-speci c TNF-α, IL-1β, and IL-6 enzyme-linked immunosorbent assay (ELISA) Kit were obtained from Bethyl Laboratories Inc., Montgomery, TX, USA. The primary antibodies of caspase-3, caspase-9, caspase-12, AFT4 (Activating transcription factor-4), AFT6α (Activating transcription factor-6α), XBP-1 (X-box binding protein 1), ERK-1/2 (Extracellular signal-regulated kinase-1/2), and P38 were purchased from Abcam, Cambridge, MA, USA.
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