Interleukin (IL)-1β levels were quantified in the retina using a rat IL-1β ELISA kit (H002, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's protocol. The concentration of IL-1β was calculated as pg IL-1β/mg total retina protein in each sample according to the manufacturer's protocol. VEGF levels were quantified in the retina using a rat VEGF ELISA kit (H044, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer's protocol. The standard solution or the samples were added in a 96-well plate that was coated with a monoclonal antibody from the kit. The samples were incubated for 2 h 37°C. After washing, the samples were detected at the wavelength of 450 nm using a VERSA max tunable microplate reader (Molecular Devices, LLC). The ELISA assay could detect concentrations of VEGF ≥15 ng/ml.
Rat il 1β elisa kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Rat IL-1β ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of rat interleukin-1 beta (IL-1β) in biological samples.
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Lab products found in correlation
2 protocols using rat il 1β elisa kit
1
Quantification of IL-1β and VEGF in Rat Retina
2
Rat Inflammation and Oxidative Stress Assay
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Main reagents and instruments included Rat IL-1β ELISA Kit (ML037361, China); Il-6 RAT ELISA Kit (ML102828, China); Rat TNF-α ELISA Kit (Ml002859, China); Rat COX-2 ELISA Kit (Ml058808, China); Rat PGE2 ELISA Kit (ML003036, China); MDA Assay Kit (A003-1-2, Nanjing Jiancheng, China); SOD Assay Kit (A001-3-2, Nanjing Jiancheng, China); CAT Assay Kit (A007-1-1, Nanjing Jiancheng, China); Rat IgG ELISA Kit (ML003019, China); Rat SIgA ELISA Kit (Ml003136, China); NGF antibody (DF6061, affinity, USA); carrageenan (C1013-25G, Merck, USA); electric constant temperature blast drying oven (DGG-9140B, Shanghai Shenxin, China); high-speed refrigerated centrifuge (Thermo Scientific, USA); microplate reader (SPECTCA MAX190, Molecular, USA); fast mixer (MX-S, SCILOGEX, USA); upright fluorescence microscope (NIKON ECLIPSE C1, Nikon, Japan).
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