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U3500

Manufactured by Hitachi
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Sourced in Japan

The U3500 is a multipurpose laboratory equipment designed for a variety of scientific applications. It features a compact and durable construction to withstand constant use in the laboratory environment.

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Lab products found in correlation

D4540 100 ml, by Merck Group (1 mentions) Cyclohexane, by Fujifilm (1 mentions) Acetonitrile, by Fujifilm (1 mentions) Perylene, by Fujifilm (1 mentions) N hexane, by Fujifilm (1 mentions)

2 protocols using u3500

1

Measuring PpIX Molar Extinction Coefficient

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The molar extinction coefficient of a PpIX solution was measured for numerical calculation. PpIX powder (P8293-1G, Sigma-Aldrich, St. Louis, Missouri) was dissolved in dimethyl sulfoxide (D4540-100ML, Sigma-Aldrich) at a concentration of 10  μM . The PpIX solution (3 mL) was added to a quartz cell (F15-UV-10, As One, Japan) with an optical path length of 10 mm. The molar extinction coefficient ε(λ) of the PpIX solution was then measured by a spectrophotometer (U3500, Hitachi, Japan).
Ihara D., Hazama H., Nishimura T., Morita Y, & Awazu K. (2020). Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study. Journal of Biomedical Optics, 25(6), 063809.
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2

Steady-state absorption spectroscopy of perylene

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Steady-state absorption spectra were recorded on a Hitachi U-3500. Perylene (FUJIFILM Wako) was used after recrystallization from ethanol. Acetonitrile (FUJIFILM Wako, infinity pure grade), n-hexane (FUJIFILM Wako, infinity pure grade), and cyclohexane (FUJIFILM Wako, for Spectrochemical Analysis) were used as received. The concentration of Perylene was typically 2.1 Â 10 À4 M. The solution sample was filled into a home-built rotation cell with 1 mm-thick quartz windows and a Teflon spacer for adjustment of optical length (0.1-2 mm thickness). During the time-resolved measurements, the rotational motion provided a fresh portion of the sample solution with individual optical pulses. All the measurements were performed at 22.5 AE 1 1C.
Sotome H., Koga M., Sawada T, & Miyasaka H. (2022). Femtosecond dynamics of stepwise two-photon ionization in solutions as revealed by pump-repump-probe detection with a burst mode of photoexcitation. Physical chemistry chemical physics : PCCP, 24(23).
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