HSPC, Chol, CHEM, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (DSPE-DTPA) were purchased from Avanti Polar Lipids Inc (Alabaster, AL). Phosphate-buffered saline (PBS) pH 7.2 (1×) was obtained from Life Technologies (Burlington, ON, Canada). Chloroform, indium (III) chloride (InCl3), sodium acetate (NaOAc), trifluoroacetic acid and tetrabutylammonium phosphate (TBAP) were purchased from Sigma-Aldrich (St Louis, MO, USA). High-performance liquid chromatography (HPLC)-grade methanol (CH3OH), acetonitrile (CH3CN) and methylene chloride (CH2Cl2) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Radionuclide 111InCl3 in 0.05 M HCl solution was supplied by Nordion Inc (Ottawa, ON, Canada). Deionized and filtered water used in experiments was obtained through a Barnstead Nanopure Infinity water system (Dubuque, IA, USA). A Waters HPLC system (Milford, MA, USA) consisting of a 1525 Binary Pump, 717+ Autosampler, 2998 Photodiode Array Detector, 474 Scanning Fluorescence Detector, as well as a PerkinElmer Radiomatic Flow Scintillation Analyzer (Waltham, MA, USA) was used to assay the fluorometric and radiometric characteristics of different liposome formulas.
Methylene chloride ch2cl2
Manufactured by Thermo Fisher Scientific
Sourced in United States
Methylene chloride (CH2Cl2) is a colorless, volatile organic compound. It is a common solvent used in various industrial and laboratory applications. Methylene chloride has a low boiling point and high solvency power, making it a useful agent for extraction, degreasing, and cleaning processes.
Automatically generated - may contain errors
Lab products found in correlation
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe peg2000, by Avanti Polar Lipids (1 mentions)
Hplc system, by Waters Corporation (1 mentions)
Chloroform, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Formic acid hcooh, by Merck Group (1 mentions)
Sodium phosphate dibasic na2hpo4, by Merck Group (1 mentions)
Sodium phosphate monobasic monohydrate nah2po4 h2o, by Merck Group (1 mentions)
2 protocols using methylene chloride ch2cl2
1
Radiolabeling Liposomes with Indium-111
2
Solid Phase Extraction of Whole Blood Samples
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Acetonitrile (ACN) of Optima LC-MS grade, methanol (MeOH), 2-propanol (C 3 H 7 OH), and methylene chloride (CH 2 Cl 2 ) of HPLC grade were obtained by Thermo Fisher Scientific (Pittsburgh, PA, USA). Hydrochloric acid (HCl), ammonium hydroxide (NH 4 OH) of Certified ACS plus grade, glacial acetic acid (CH 3 COOH) of HPLC grade and sodium hydroxide (NaOH) of analytical purity grade were also supplied from Thermo Fisher Scientific (Pittsburgh, PA, USA). Formic acid (HCOOH) of LC-MS grade, sodium phosphate dibasic (Na 2 HPO 4 ), and sodium phosphate monobasic monohydrate (NaH 2 PO 4 •H 2 O) were ob- tained from Sigma Aldrich (St. Louis, MO, USA). HPLC-grade water was purchased by Aurora Corporation (Wilmington, DE, USA). The solid phase extraction (SPE) experiments were performed by using a positive pressure manifold of 48 positions purchased from UCT (Bristol, PA, USA) and Clean Screen ® DAU SPE Cartridges (200 mg, 10 mL). Blank whole blood and premortem and postmortem whole blood samples from real toxicological cases were obtained according to the rules, ethics, and procedures of the Laboratory of Toxicology, Division of Forensic Science, Department of Safety and Homeland Security, State of Delaware, Wilmington, DE, USA.
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