In order to check whether CSEP080 and VviB6f influenced plant photosynthesis, CSEP080 and VviB6f were expressed in the tobacco leaves and plant photosynthetic rate was measured. The tobacco leaf tissues were injected by agrobacterium carrying CSEP080 or VviB6f, and then resuspended by the prepared solution (10 mM MgCl2, 10 mM acetosyringone). The plant photosynthesis capacity parameters, including intercellular CO2 concentration, stomatal conductance, photosynthesis rate and transpiration rate, were measured after 3 days of the injection with the CIRAS-3 system (PPSYSTEMS, 718 USA), following the manufacturer’s instructions and software.
Ciras 3 system
Manufactured by PP Systems
Sourced in United States
The CIRAS-3 system is a gas exchange measurement instrument designed for plant physiology research. It measures the exchange of carbon dioxide and water vapor between plants and the surrounding environment, providing data on photosynthesis, respiration, and transpiration rates.
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Lab products found in correlation
4 protocols using ciras 3 system
1
Evaluating CSEP080 and VviB6f Effects on Photosynthesis
2
Photosynthetic Efficiency under AquaDust Treatment
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Rates of transpiration , assimilation , and stomatal conductance were collected from regions infiltrated with AquaDust using a CIRAS-3 system (PPSystems, Amesbury, MA, USA). Measurements were taken after gas exchange parameters stabilized (typically in 2 to 3 min). Cuvette parameters were of 300 cc min-1 gas flow rate, and temperature, light levels, and VPD were set to match the ambient conditions in the growth chamber as described earlier in the Materials and Methods section on growing conditions, or as indicated in captions. The AquaDust spectrum was collected immediately after the gas exchange was performed on the corresponding region.
3
Photosynthesis Measurements in Leaf Canopy
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Photosynthesis measurements were performed on five leaves randomly selected near the base (hereafter referred to as bottom leaves, Figure S1 ) and five more near the top (hereafter referred to as top leaves, Figure S1 ). Measured gas-exchange parameters included net photosynthesis rate (A), stomatal conductance (gs), transpiration rate (E), and leaf intercellular CO2 concentration (Ci). These parameters were all measured using a portable photosynthetic CIRAS-3 system (PP Systems, Amesbury, MA, USA) [34 (link)].
4
Soil Properties and Environmental Conditions
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After treatment application (30 July), samples of soils were collected at a depth of 20 cm. A cylindrical sample of 378.67 cm 3 of bulk soil per sample was used to determine the soil bulk density and the soil water content (SWC). Soil samples were weighted and dried in an oven at 70 ℃. Dried samples were used for determining the SWD and BD according to the following equations: 20 grams of soil samples diluted in deionized water (2:5, w:v) were used for determining the soil pH with an autotitrator (Omnis titrator, Methohm, Switzerland). Soil relative humidity (RH), temperature and water evaporation from soils were measured in situ with a Soil Respiration Chamber (SRC-2) coupled to Ciras-3 system (PP Systems, Amesbury, MA, USA) at noon on June 9. The SRC-2 chamber was stabilized for one minute and then, parameters were recorded.
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