Electrostatic field orbital trap mass spectrometer

The following equipment was used: 3000-Da nanofiltration membrane (SJM, Hefei, China), AB-8 macroporous resin column (Bonc, Cangzhou, China), ordinary-phase silica gel column (200–300 mesh, Anhui Liangchen Silicon Material Co. Ltd., Huoshan, China) and a Varian Prostar HPLC instrument (Model 325) (Varian, Mulgrave, Australia). The HPLC purifications were run on Agilent 1260 HPLC (Agilent, Palo Alto, CA, USA). IR (infrared) spectra were recorded on Nicolet iN10 (Thermo Scientific Instrument Co., Boston, MA, USA) with KBr pellets. NMR spectra were measured on an AVANCE III (600 MHz) spectrometer (Bruker, Fallanden, Switzerland) using methanol-d4 as solvent g methanol-d4 (Sigma-Aldrich, St. Louis, MO, USA). HR-ESI-MS were determined on an Electrostatic Field Orbital Trap Mass Spectrometer (Thermo Scientific, Bremen, Germany) using an ESI source.

HPLC was run on Agilent 1260 HPLC (Agilent, Palo Alto, CA, USA). IR (infrared) spectra was recorded on Nicolet iN10 (Thermo Scientific Instrument Co., Boston, MA, USA) with KBr pellets. NMR spectra was measured on an AVANCE III (600 MHz) spectrometer (Bruker, Fallanden, Switzerland) using methanol-d₄ (Sigma-Aldrich St. Louis, MO, USA) as solvent. HR-ESI-MS were determined on an electrostatic field orbital trap mass spectrometer (Thermo Scientific, Bremen, Germany) using an ESI source.