Deionized h2o

Manufactured by Merck Group

Sourced in Germany

Deionized H2O is a highly purified form of water, where all ions have been removed through a process of deionization. It is a colorless, odorless, and tasteless liquid that serves as a core laboratory reagent, providing a consistent and controlled water source for various scientific applications.

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Lab products found in correlation

4 protocols using deionized h2o

Phytochemical Analysis and Antioxidant Assays

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Acetone, sodium carbonate (Na₂CO₃), and sodium hydroxide (NaOH) were purchased from J.T.Baker, Deventer, Netherlands. ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), aluminum chloride (AlCl₃), catechin, ethanol p.a. (99.5%), Folin-Ciocalteau 1 N, gallic acid, sodium nitrite (NaNO₂), potassium persulfate (K₂S₂O₈) and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were obtained from Sigma-Aaldrich, Steinheim, Germany. Deionized H₂O was purchased from Millipore, Bedford, MA, USA.

Roselló-Soto E., Barba F.J., Putnik P., Bursać Kovačević D., Lorenzo J.M, & Cantavella-Ferrero Y. (2018). Enhancing Bioactive Antioxidants’ Extraction from “Horchata de Chufa” By-Products. Foods, 7(10), 161.

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Polyphenols Extraction and Characterization

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Polyphenol standards (purity > 98%) were purchased as follows: 4-CQA, 3,4-diCQA, p-coumaric acid, quinic acid, ferulic acid, caffeic acid, and gallic acid from Sigma-Aldrich (Milan, Italy). The reagents used in simulated GiD, including α-amylase from human saliva, protease from Streptomyces griseus (Pronase), pepsin from porcine gastric mucosa, bile salts, Viscozyme L, pancreatin from porcine pancreas, potassium chloride (KCl), sodium chloride (NaCl), potassium dihydrogen phosphate (KH₂PO₄), sodium bicarbonate (NaHCO₃), magnesium chloride hexahydrate (MgCl₂(H₂O)₆), ammonium carbonate (NaHCO₃)₂), sodium hydroxide (NaOH), and calcium chloride dihydrate (CaCl₂(H₂O)₂), were provided from Sigma-Aldrich (Milan, Italy). The standards used in antioxidant assays, including 2,3,5-triphenyltetrazolio chloride (TPTZ), Trolox, 2’2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), potassium persulfate (K₂S₂O₈), and ferric chloride (FeCl₃), were acquired from Sigma-Aldrich (Milan, Italy).
Formic acid (FA), water (H₂O), and methanol (MeOH) were provided from Carlo Erba reagents (Milan, Italy). Deionized H₂O was obtained from a Milli-Q water system (Millipore, Darmstadt, Germany).

Castaldo L., Lombardi S., Gaspari A., Rubino M., Izzo L., Narváez A., Ritieni A, & Grosso M. (2021). In Vitro Bioaccessibility and Antioxidant Activity of Polyphenolic Compounds from Spent Coffee Grounds-Enriched Cookies. Foods, 10(8), 1837.

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Quantifying Surface Wettability with OCA 15Pro

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SWCA measurements were performed on an OCA 15Pro contact angle system (DataPhysics Instruments). Data acquisition and evaluation were realized with SWCA 20—contact angle (DataPhysics Instruments, version 2.0). For quantifying an average Young’s contact angle (θγ), 2 µL deionized H₂O (18.2 M Ωcm at 25 °C, Merck Millipore) was dispensed with a rate of 0.2 µLs⁻¹ from a 500-µL Hamilton syringe onto the sample surface. After allowing the droplet to settle for ∼3 s, an image was acquired for further processing. The procedure was repeated at least three times on different spots on the surface, and the SD was calculated.

Liu K.S., Henning A., Heindl M.W., Allert R.D., Bartl J.D., Sharp I.D., Rizzato R, & Bucher D.B. (2022). Surface NMR using quantum sensors in diamond. Proceedings of the National Academy of Sciences of the United States of America, 119(5), e2111607119.

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Preparation of Reagents and Samples

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All chemicals for matrix and solvent preparation were of pro-analysis grade and obtained from Sigma-Aldrich (Stockholm, Sweden), unless otherwise specified. The TissueTek optimal cutting temperature (OCT) compound was purchased from Sakura Finetek (AJ Alphen aan den Rijn, The Netherlands). Deionized H₂O was obtained from a MilliQ purification system (Merck Millipore, Darmstadt, Germany).

Kaya I., Jennische E., Lange S, & Malmberg P. (2018). Dual polarity MALDI imaging mass spectrometry on the same pixel points reveals spatial lipid localizations at high-spatial resolutions in rat small intestine †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8ay00645h. Analytical Methods, 10(21), 2428-2435.

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