Dawn heleos 2 multiangle static light scattering mals detector

Manufactured by Wyatt Technology

The Dawn Heleos II is a multiangle static light scattering (MALS) detector manufactured by Wyatt Technology. It measures the intensity of scattered light at multiple angles to determine the molar mass and size of macromolecules and nanoparticles in solution.

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Lab products found in correlation

Wyatt astra 5 software, by Wyatt Technology (1 mentions) Superdex 200 10 30 gl size exclusion column, by GE Healthcare (1 mentions) Superdex 200 5 150 gl column, by GE Healthcare (1 mentions) Optilab t rex refractive index detector, by Agilent Technologies (1 mentions)

2 protocols using dawn heleos 2 multiangle static light scattering mals detector

Size Exclusion Chromatography for Protein Analysis

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Size exclusion chromatography–multiangle light scattering (SEC-MALS) was performed as previously described (Klebba et al., 2015b ). Briefly, Asl mutants (50 μM, 100 μl) were injected onto a Superdex 200 10/30 GL size exclusion column (GE Healthcare Life Sciences) at 0.5 ml/min in buffer (25 mM HEPES, pH 7.5, 300 mM NaCl, 0.1% β-mercaptoethanol, and 0.2 g/l sodium azide). Eluent was analyzed by a tandem RI detector/Dawn Heleos II multiangle static light scattering (MALS) detector (Wyatt Technology). Light scattering and refractive index data were used to calculate the weight-averaged molar mass using Wyatt Astra V software (Wyatt Technology).

Boese C.J., Nye J., Buster D.W., McLamarrah T.A., Byrnes A.E., Slep K.C., Rusan N.M, & Rogers G.C. (2018). Asterless is a Polo-like kinase 4 substrate that both activates and inhibits kinase activity depending on its phosphorylation state. Molecular Biology of the Cell, 29(23), 2874-2886.

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SEC-MALS Analysis of EPO-EPOR Interactions

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This was performed essentially as described (Jenni et al., 2015 (link)). SEC-MALS was carried out on
a Dawn Heleos-II Multi-Angle static Light Scattering (MALS) detector (Wyatt)
with the Optilab T-Rex refractive index detector (Agilent Technologies)
after passing through a Superdex 200 5/150 GL column (GE Healthcare Life
Sciences). A total of 15 μl of 40 μM EPO, EPO R150Q, or 38
μM EPO receptor extracellular region (EPOR) was injected. For the
1:1 ratios (Ligand : EPOR), 29 μM : 29 μM was injected.
Fitting was done with Astra software using a dn/dc value of 0.179, which
assumes 14% carbohydrate by mass for each protein.

Kim A.R., Ulirsch J.C., Wilmes S., Unal E., Moraga I., Karakukcu M., Yuan D., Kazerounian S., Abdulhay N.J., King D.S., Gupta N., Gabriel S.B., Lander E.S., Patiroglu T., Ozcan A., Ozdemir M.A., Garcia K.C., Piehler J., Gazda H.T., Klein D.E, & Sankaran V.G. (2017). Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation. Cell, 168(6), 1053-1064.e15.

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