Rabbit anti p lrp1

Paraffin-embedded livers (n=3–5 per time point) were sectioned into 4-μm thick slices. Standard immunohistochemistry was performed using anti-α-SMA (1:50), anti-Collagen I (1:100), and anti-fibrinogen (1:50) primary antibodies with an avidin–biotin complex-horseradish peroxidase developer. Double staining on sections used mouse anti-α-SMA (1:50) and rabbit anti-p-LRP1 (1:200) primary antibodies and Qdot-conjugated Q705 and Q605 secondary antibodies, respectively (Life Technologies). Triple staining was accomplished by adding goat anti-t-PA (1:150) labeled with biotinylated secondary and Streptavidin-Q655. Specificity of the α-SMA was confirmed using a mouse-on-mouse blocking kit (Vector Laboratories, Burlingame, CA, USA). Sequential double labeling of 4-μm thick OCT sections from LRP^flox/flox; SM22-cre ^{+/− or −/−}, and t-PA^{+/+ or −/−} mice (1:1 acetone:MeOH fix) used anti-LRP1 (1:50, American Diagnostica) with a Cy5-conjugated secondary antibody, followed by Cy3-conjugated anti-α-SMA (1:1000, Sigma-Aldrich).