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6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid

Manufactured by Roche
Sourced in Germany

6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid is a chemical compound. It is a white crystalline solid. The compound has a molecular formula of C18H24O3.

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2 protocols using 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid

1

Antioxidant and Oxidative Stress Assays

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ABTS [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)], 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) was purchased from Roche diagnostics (Mannheim, Germany) and Fluka (Buchs, Switzerland), respectively. Nitro blue tetrazolium (NBT), 2-deoxy-2-ribose, sodium nitroprusside (SNP), phenazine methosulfate (PMS), 1,10-phenanthroline, 5,5′-dithiobis-2-nitrobenzoic acid (DTNB), lipoic acid, ferrozine and bathophenanthrolinesulfonate disodium salt were purchased from Sisco Research Laboratories Pvt. Ltd, Mumbai, India. Folin-ciocalteu reagent, 2,4-dinitrophenylhydrazine (DNPH), butylated hydroxyltoluene (BHT), 1-chloro-2,4-dinitrobenzene (CDNB), chloramine-T, Dimethyl-4-aminobenzaldehyde and hydroxylamine hydrochloride were taken from Merck, Mumbai, India. Spectrochem Pvt. Ltd, (India) supplied Diethylenetriaminepentaacetic acid (DTPA). Iron-dextran and guanidine hydrochloride was obtained from Sigma-Aldrich, USA. Desirox (Deferasirox) was purchased from Cipla Ltd., India. All the other used reagents are molecular biology grade and obtained from reputed suppliers.
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2

Analytical Grade Chemical Procurement

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All chemicals used were of analytical grade. Methanol, ethanol and acetic acid were purchased from Merck (Darmstadt, Germany); potassium persulfate (dipotassium peroxodisulfate), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Hoffman-La Roche. 2,20-azinobis (3-ethylben-zothiazoline-6-sulfonic acid) diammonium salt (ABTS), gallic acid, Folin-Ciocalteu's phenol reagent (product F9252), were purchased from Sigma-Aldrich.
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