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H2o2 determination kit

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The H2O2 determination kit is a laboratory equipment designed to quantify the concentration of hydrogen peroxide (H2O2) in a sample. It provides a reliable and efficient method for analyzing the presence and amount of H2O2 in various types of samples.

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Lab products found in correlation

Smz18, by Nikon (2 mentions)

2 protocols using h2o2 determination kit

1

Detecting Hydrogen Peroxide in Transgenic Plants

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Detection of H2O2 in leaves was conducted using diaminobenzidine (DAB) staining; OsSHMT transgenic A. thaliana and its wild type were treated at 4 °C for 12, 24 and 36 h. The treatment was repeated three times in the same growth chamber. Leaves from these two lines were sampled and immersed in 50 mg/L DAB solution by vacuum-pumping for 1 h and incubated overnight at room temperature. These leaves were then decolourised with 95% ethanol in a water bath at 80 °C, and the reddish brown spots in the transgenic leaves and wild-type leaves were observed using an integrated microscope (Nikon, SMZ18). The H2O2 content in the 12 h-treated leaves of the transgenic line and the wild type were determined using an H2O2 determination kit (Solarbio Life Sciences).
Fang C., Zhang P., Li L., Yang L., Mu D., Yan X., Li Z, & Lin W. (2020). Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. BMC Plant Biology, 20, 236.
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2

Quantifying Leaf H2O2 Levels by DAB Staining

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Detection of H 2 O 2 in leaves was conducted using DAB staining; OsSHMT transgenic A. thaliana and its wild type were treated at 4 °C for 12, 24 and 36 h. The treatment was repeated three times in the same growth chamber. Leaves from these two lines were sampled and immersed in 50 mg/L diaminobenzidine (DAB) solution by vacuum-pumping for 1 h and incubated overnight at room temperature. These leaves were then decolourised with 95% ethanol in a water bath at 80 °C, and the reddish brown spots in the transgenic leaves and wild-type leaves were observed using an integrated microscope (Nikon, SMZ18).
The H 2 O 2 content in the 12 h-treated leaves of the transgenic line and the wild type were determined using an H 2 O 2 determination kit (Solarbio Life Sciences).
Fang C., Zhang P., Li L., Yang L., Mu D., Yan X., Li Z., & Lin W. (2020). Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance.
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