400 nmr spectrometers

Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen. Molecular weight protein markers were purchased from Thermo Scientific. TNFα was purchased from Immuno Tools (Friesoythe, Germany). Cocktail protease inhibitors were purchased from Roche (Mannheim, Germany). Reporter lysis buffer and luciferin were purchased from Promega Corporation (Madison, WI, USA). All other solvents and chemicals including dexamethasone (DEX) and MG-132 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Silica gel 60 (70–230 mesh), Celite^® 545 particle size 0.02–0.1 mm, CAS 68855-54-9, pH 10 (100 g/l, H₂O, 20 °C), neutral alumina Alugram^®, and RP C-18 silica gel used for chromatography were purchased from Macherey-Nagel (Düren, Germany). ¹H (400 MHz) spectra were measured on Bruker 400 NMR spectrometers. Chemical shifts were referenced to the residual solvent signal (CDCl₃: δ_H = 7.26). Purifications were monitored by TLC on Merck 60 F254 (0.25 mm) plates, visualized by staining with 5% H₂SO₄ in EtOH and heating. Chios mastiha tree leaves were kindly provided by Chios Mastic Growers Association and Mastiha Shop.

Dulbecco’s modified Eagle medium (DMEM) (4.5 g/L glucose), fetal bovine serum (FBS), and molecular weight protein markers were obtained from Thermo Fischer Scientific (GmbH, Frankfurt, Germany). Recombinant Human Tumor Necrosis Factor alpha (TNFα) was obtained from Peprotech (London, UK). A cocktail of protease inhibitors was obtained from Roche (Mannheim, Germany). Reporter lysis buffer and luciferin were purchased from Promega Corporation (Madison, WI, USA). For chromatography studies, silica gel 60, Celite^® 545 particle size 0.02–0.1 mm, CAS 68855-54-9, pH 10 (100 g/L, H₂O, 20 °C), neutral alumina Alugram^®, and RP C-18 silica gel were used and obtained from Macherey-Nagel (Düren, Germany). 1 H (400 MHz) spectra were measured on Bruker 400 NMR spectrometers. Chemical shifts were referenced to the residual solvent signal (CDCl3: δH = 7.26). Purifications were monitored by TLC on Merck 60 F254 (0.25 mm) plates, visualized by staining with 5% H₂SO₄ in EtOH and heating. HPLC JASCO Hichrom, 250 × 25 mm, silica UV−vis detector-2075 plus (Oklahoma, Japan) was used. Dexamethasone (DEX) and all the other solvents and chemicals used were obtained from Sigma-Aldrich (St. Louis, MO, USA). Finally, the Chios Mastiha was a kind donation from the Mastiha Shop and the Chios Mastic Growers Association.