Kanamycin sulphate

Mix (Green) for PCR amplification and pDE2 Directional TOPO Expression Kit for expression vector construction were purchased from TsingKe (Beijing, China). The Universal DNA Purification Kit used for PCR product purification was purchased from TianGen (Beijing, China). Escherichia coli Trans5ɑ and BL21(DE3) were purchased from TransGen Biotech (Beijing, China) and used for gene cloning and protein expression, respectively. Sonics Vibra-Cell™ used for cell disruption was purchased from Sonics & Materials (Newtown, USA). Ni-NTA Sefinose™ Resin was obtained from Sangon Biotech (Shanghai, China) for protein purification. Amicon® Ultra-15 Centrifugal Filter Devices (Merck Millipore, Germany) were used for protein concentration. NanoDrop 2000c (Thermo Fisher Scientific, Waltham, MA) was used to analyze the concentrations of DNA and protein. High-performance anion-exchange chromatography (HPAEC) (Dionex ICS-5000) with a pulsed amperometric detector (PAD) was obtained from Thermo Fisher Scientific (Waltham, MA). Kanamycin sulphate, isopropylthio-β-galactoside (IPTG), imidazole, and other chemical reagents used in this study were purchased from yuanye Bio-Technology (Shanghai, China).

Escherichia coli Trans5ɑ and BL21(DE3) (TransGen Biotech, Beijing, China) was used for plasmid maintenance and protein expression, respectively. T4 DNA ligase, NdeI, HindIII (New England Biolabs, Ipswich, MA, USA) and pET-28a + vector (Invitrogen, Carlsbad, CA, USA) were used for gene cloning. Ni-NTA Sefinose™ Resin was purchased from Sangon Biotech (Shanghai, China) for protein purification. p-Nitrophenyl-α-D-glucopyranoside (pNPαG), p-Nitrophenyl-β-D-glucopyranoside (pNPβG), p-Nitrophenyl-β-D-xylopyranoside (pNPβX), p-Nitrophenyl-α-D-mannopyranoside (pNPαM), and p-Nitrophenyl-α-L-arobinopyranoside (pNPαA) were from J&K Scientific (Beijing, China), while soluble starch, malto-oligosaccharides, Nigerose, Kojibiose, Isomaltose, Sucrose, Kanamycin sulphate, imidazole, isopropylthio-β-galactoside (IPTG) and other reagents were purchased from yuanye Bio-Technology (Shanghai, China).