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2 protocols using sodium dihydrogen phosphate

1

Antioxidant Profiling of Plant Compounds

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Ethanol, sodium carbonate (Na2CO3), Disodium hydrogenorthophosphate, sodium dihydrogen phosphate, 30% hydrogen peroxide, Vit C, catechol, 2-methoxyphenol, β-mercaptoEthanol, polyvinylpyrrolidone (PVPP), glycerol, tris-base, hydrochloric acid, and magnesium chloride (MgCl2) were obtained from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Folin–Ciocalteu reagent, potassium persulfate, leupeptin, phenylmethylsulfonyl fluoride, boracic acid, borax, and l-phenylalanine were purchased from Beijing Solarbio Science and Technology CO., Ltd. (Beijing, China). Gallic acid, hydroxybenzoic acid, chlorogenic acid, caffeic acid, sinapic acid, ferulic acid, rutin, cinnamic acid, catechin, and quercetin, were obtained from Shanghai Yuanye Biochemical Co., Ltd. (Shanghai, China). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) and adenosine triphosphate were obtained from Shanghai Aladdin Biochemical Co., Ltd. (Shanghai, China). Methyl jasmonate and mEthanol were obtained from Sigma Chemical Co. (St. Louis, MO, USA). MEthanol was reagent of HPLC-grade. The other chemical reagents were of reagent grade.
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2

HPLC Quantification of Epalrestat Purity

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The active pharmaceutical ingredient of epalrestat (purity > 99.5%) was obtained from Ningxia Kangya Pharmaceutical Industry (China). Acetonitrile and methanol of HPLC grade were purchased from Fisher (Fair lawn, NJ, USA). N,N-dimethyl formamide was obtained from Shandong Yuwang Industrial Co., Ltd (Shandong, China). Ammonium acetate (HPLC grade) was obtained from Dikma (Richmond Hill, NY, USA). Sodium dihydrogen phosphate, potassium phosphate monobasic and sodium phosphate dibasic dodecahydrate were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd (Tianjin, China). Hydrochloric acid was obtained from Tianjin Kaixin Chemical Reagent Manufacturing Co., Ltd (Tianjin, China). H2O2 (30%), sodium hydroxide and sodium chloride were purchased from Tianjin Hengxing Chemical Reagent Manufacturing Co., Ltd (Tianjin, China). Ultra-pure water from Wahaha (Hangzhou, Zhejiang, China) was used. All other reagents and chemicals used were of analytical grade and are commercially available.
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