Lipidure coat plate a u96

Manufactured by NOF corporation

Sourced in Japan

LIPIDURE®-COAT PLATE A-U96 is a 96-well non-treated microplate coated with LIPIDURE®, a hydrophilic polymer. The LIPIDURE® coating helps to reduce non-specific binding of proteins, peptides, and other biomolecules to the surface of the microplate wells.

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Lab products found in correlation

Eclipse e400 microscope, by Nikon (15 mentions)

7 protocols using lipidure coat plate a u96

Spheroids Generation using LIPIDURE-COAT PLATE

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Spheroids were generated using LIPIDURE-COAT PLATE A-U96 (NOF Corporation, Tokyo, Japan) according to the manufacturer’s instruction and as described previously [48 (link)].

La Monica S., Fumarola C., Cretella D., Bonelli M., Minari R., Cavazzoni A., Digiacomo G., Galetti M., Volta F., Mancini M., Petronini P.G., Tiseo M, & Alfieri R. (2020). Efficacy of the CDK4/6 Dual Inhibitor Abemaciclib in EGFR-Mutated NSCLC Cell Lines with Different Resistance Mechanisms to Osimertinib. Cancers, 13(1), 6.

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Spheroid Formation and Drug Testing

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Spheroids were generated using LIPIDURE^®-COAT PLATE A-U96 (NOF Corporation, Tokyo, Japan) as previously described [40 (link)]. Briefly, 500 cells were seeded in medium with 0.2% FCS and after 2 days (T0) the spheroids were treated with 1μM NVP-BGJ398 in the presence of FGF2 for further 7 days. The effect of the drug was evaluated in term of volume changes using the Nikon Eclipse E400 Microscope with digital Net camera. The volume of spheroids was measured [D=(Dmax+Dmin)/2; V=4/3π(D/2)3] with Image J software and the Fold Increase (FI) index was calculated as the ratio between the spheroid volume after 7 days and the volume at T0.

Fumarola C., Cretella D., La Monica S., Bonelli M.A., Alfieri R., Caffarra C., Quaini F., Madeddu D., Falco A., Cavazzoni A., Digiacomo G., Mazzaschi G., Vivo V., Barocelli E., Tiseo M., Petronini P.G, & Ardizzoni A. (2017). Enhancement of the anti-tumor activity of FGFR1 inhibition in squamous cell lung cancer by targeting downstream signaling involved in glucose metabolism. Oncotarget, 8(54), 91841-91859.

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Spheroid-based drug evaluation

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Spheroids from MSTO-211H were generated using LIPIDURE®-COAT PLATE A-U96 (NOF Corporation, Japan) according to manufacturer's instruction. Briefly, 200 cells were seeded and after 1–3 days the spheroids were formed and treated with drugs or vehicle for 96 hours. The effects of the drugs were evaluated in terms of volume changes as previously described [16] (link).

Bonelli M.A., Digiacomo G., Fumarola C., Alfieri R., Quaini F., Falco A., Madeddu D., La Monica S., Cretella D., Ravelli A., Ulivi P., Tebaldi M., Calistri D., Delmonte A., Ampollini L., Carbognani P., Tiseo M., Cavazzoni A, & Petronini P.G. (2017). Combined Inhibition of CDK4/6 and PI3K/AKT/mTOR Pathways Induces a Synergistic Anti-Tumor Effect in Malignant Pleural Mesothelioma Cells. Neoplasia (New York, N.Y.), 19(8), 637-648.

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Generating Spheroids for Drug Evaluation

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Spheroids from HCC cells were generated using LIPIDURE^®-COAT PLATE A-U96 (NOF Corporation, Japan) according to the manufacturer’s instructions. Briefly, 3 days after seeding, the spheroids were formed and treated with drugs or vehicles for 6 or 12 days. The effects of the drugs were evaluated in terms of volume changes using the Nikon Eclipse E400 Microscope with digital Net camera. The volume of spheroids [D=(Dmax+Dmin)/2; V=4/3π(D/2)3] was measured using SpheroidSizer, a MATLAB-based and open-source software application (24 (link)).

Digiacomo G., Fumarola C., La Monica S., Bonelli M., Cavazzoni A., Galetti M., Terenziani R., Eltayeb K., Volta F., Zoppi S., Bertolini P., Missale G., Alfieri R, & Petronini P.G. (2022). CDK4/6 inhibitors improve the anti-tumor efficacy of lenvatinib in hepatocarcinoma cells. Frontiers in Oncology, 12, 942341.

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Spheroid-Based Drug Efficacy Evaluation

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Spheroids were generated using LIPIDURE®-COAT PLATE A-U96 (NOF Corporation, Tokyo, Japan) as previously described (28 (link)). Briefly, 500 cells were seeded in RPMI 1,640 medium and after 3 days (T0) the spheroids were treated with BGJ398, FIIN-2 or UPR1376 for further 10 days. The effect of the drugs was evaluated in term of volume changes using the Nikon Eclipse E400 Microscope with digital Net camera. The volume of spheroids was measured [D = (Dmax+Dmin)/2; V = 4/3π(D/2)3] using SpheroidSizer, a MATLAB-based and open-source software application (29 (link)).

Fumarola C., Bozza N., Castelli R., Ferlenghi F., Marseglia G., Lodola A., Bonelli M., La Monica S., Cretella D., Alfieri R., Minari R., Galetti M., Tiseo M., Ardizzoni A., Mor M, & Petronini P.G. (2019). Expanding the Arsenal of FGFR Inhibitors: A Novel Chloroacetamide Derivative as a New Irreversible Agent With Anti-proliferative Activity Against FGFR1-Amplified Lung Cancer Cell Lines. Frontiers in Oncology, 9, 179.

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Evaluating Drug Effects on HCC Spheroids

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Spheroids from HCC cells were generated using LIPIDURE^®-COAT PLATE A-U96 (NOF Corporation, Japan) according to manufacturer's instruction. Briefly, 3 days after seeding the spheroids were formed and treated with drugs or vehicle for 4 or 8 days. The effects of the drugs were evaluated in terms of volume changes using the Nikon Eclipse E400 Microscope with digital Net camera. The volume of spheroids [D = (Dmax + Dmin)/2; V = 4/3π (D/2)3] was measured using SpheroidSizer, a MATLAB-based and open-source software application (26 (link)).

Digiacomo G., Fumarola C., La Monica S., Bonelli M.A., Cretella D., Alfieri R., Cavazzoni A., Galetti M., Bertolini P., Missale G, & Petronini P.G. (2020). Simultaneous Combination of the CDK4/6 Inhibitor Palbociclib With Regorafenib Induces Enhanced Anti-tumor Effects in Hepatocarcinoma Cell Lines. Frontiers in Oncology, 10, 563249.

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Spheroid Assay for Drug Screening

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Spheroids from transfected clones were generated using LIPIDURE^®-COAT PLATE A-U96 (NOF Corporation, Tokjo, Japan) according to manufacturer's instruction. Briefly, 500 cells were seeded and after 1–3 days the spheroids were treated with vehicle or drugs until 14 days. The effects of the drugs were evaluated in term of volume changes using the Nikon Eclipse E400 Microscope with digital Net camera. The volume of the spheroids (V) was calculated as volume of spheres having as diameter the average of the maximum and minimum diameters [D=(Dmax+Dmin)/2; V=4/3π(D/2)³] obtained by measurement with Image J software [15 (link)].

Cavazzoni A., La Monica S., Alfieri R., Ravelli A., Van Der Steen N., Sciarrillo R., Madeddu D., Lagrasta C.A., Quaini F., Bonelli M., Fumarola C., Cretella D., Digiacomo G., Tiseo M., Peters G.J., Ardizzoni A., Petronini P.G, & Giovannetti E. (2017). Enhanced efficacy of AKT and FAK kinase combined inhibition in squamous cell lung carcinomas with stable reduction in PTEN. Oncotarget, 8(32), 53068-53083.

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