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Kms11

Manufactured by American Type Culture Collection
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Sourced in United States, Germany

The KMS11 is a laboratory centrifuge designed for general-purpose benchtop applications. It features a compact design and can accommodate rotors for common microcentrifuge tubes and other small sample containers. The KMS11 provides controlled speed and time settings to enable efficient separation of samples.

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Lab products found in correlation

Rpmi8226, by American Type Culture Collection (23 mentions) Mm 1s, by American Type Culture Collection (16 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) L glutamine, by Thermo Fisher Scientific (4 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Oci my5, by American Type Culture Collection (4 mentions) Mm 1r, by American Type Culture Collection (4 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Anbl 6, by American Type Culture Collection (3 mentions) Opm 2, by Leibniz Institute DSMZ (3 mentions) Rpmi 1640 medium, by Merck Group (3 mentions) Rpmi 1640 media, by Corning (2 mentions) Jp11646, by Novartis (2 mentions) Recombinant il 6, by R&D Systems (2 mentions) Azd1208, by AstraZeneca (2 mentions) Lgb321, by Novartis (2 mentions) Rpmi 1640 medium, by Corning (2 mentions) Jurkat, by American Type Culture Collection (2 mentions)

33 protocols using kms11

1

Inhibition of Aminopyrimidine Kinase in Multiple Myeloma

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The aminopyrimidine kinase inhibitor JP11646 and other members of its family of inhibitors (Table 1), LGB321 (Novartis, Berkeley, CA) and AZD1208 (Astra Zeneca, London, UK) were synthesized and obtained from Jasco Pharmaceuticals (Woburn, MA) and were used as 10 mM stocks in DMSO. The MM cell lines MM1.S (gift from Dr. Stephen Rosen, Chicago, IL), RPMI8226, U266 and KMS11 (ATCC, Manassas, VA), were maintained in RPMI 1640 media (Mediatech Inc, Manassas, VA) containing 10% FBS (Hyclone Inc., Logan, UT), 1000 U/ml penicillin/streptomycin, 4 mM L-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate. Recombinant IL-6 was purchased from R&D Systems (Minneapolis, MN).
Nair J.R., Caserta J., Belko K., Howell T., Fetterley G., Baldino C, & Lee K.P. (2016). Novel inhibition of PIM2 kinase has significant anti-tumor efficacy in multiple myeloma. Leukemia, 31(8), 1715-1726.
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2

Epigenetic Modulators in Hematological Cancers

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Human cancer cell lines K562, KMS11 and MM1S were purchased from ATCC. Antibodies were obtained from the following sources: EP300 (C-20) sc-585 from Santa Cruz, CREBBP (A-22) sc-369 from Santa Cruz, H3K27ac ab4729 from Abcam, MYC (N-262) sc-764 from Santa Cruz, GATA1 (N6) sc-265 from Santa Cruz, ACTB (Ac-15) A5441 from Sigma-Aldrich, PARP 9542 and Caspase3 9662 from Cell Signaling. CBP30 and I-CBP112 were purchased from Tocris Bioscience. C646 was purchased from Sigma-Aldrich. JQ1 was purchased from Selleck Chemicals. GNE-272 and CPI644 were synthesized in house. A-485 was obtained from the Structural Genomics Consortium.
Garcia-Carpizo V., Ruiz-Llorente S., Sarmentero J., Graña-Castro O., Pisano D.G, & Barrero M.J. (2018). CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation. Epigenetics & Chromatin, 11, 30.
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3

Isolation and Culture of Primary Myeloma Cells

Cited 1 time
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Primary MM cells were isolated from bone marrow aspirates of MM patients, using Ficoll-Hypaque density gradient sedimentation followed by CD138 microbeads separation (Miltenyi Biotec), with informed patient consent and the Research Ethics Board approval at City of Hope (IRB 15150). Normal B lymphocytes from healthy donor PBMCs were enriched by Mojosort human CD19+ cell selection kit (Biolegend) according to the manufacture manual. Purify was validated by flow cytometry of CD19 staining. Human myeloma cell lines MM1S, H929, KMS11, and RPMI8226 were obtained from ATCC. Lenalidomide resistant cell line MMR10R was a kind gift from Dr. R Z Orlowski (M.D. Anderson cancer cell, TX, USA) [28 (link)]. All myeloma cell lines and primary CD138+ MM cells were cultured in RPMI1640 medium (Corning) with 10% heat-inactivated FCS (Omega Scientific, Inc.), 2 mmol/L l-glutamine, and 1% antibiotic-antimycotic (Life Technologies). Mycoplasma was routinely tested using Mycoplasma PCR detection kit (G238, Abcam).
Du L., Liu W., Pichiorri F, & Rosen S.T. (2022). SUMOylation inhibition enhances multiple myeloma sensitivity to lenalidomide. Cancer Gene Therapy, 30(4), 567-574.
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4

Cell Lines for MM and DLBCL Research

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MM cell lines were obtained from the following sources: KMS18 (Dr. L Bergsagel, Mayo Clinic, AZ), RPMI-8226 (ATCC), U266, L363, KMS11, JJN3 (Dr. M. Kuehl, NCI, MD), MM.1S (Dr. S. Rosen, City of Hope, CA), KMS18, KMS21BM, KMS12BM, KMS12PE and KMS27 from JCRB Japanese Collection of Research Bioresources and KARPAS-620, OCI-MY5 and H1112 from Jonathan Keats, TGEN, City of Hope affiliate. DLBCL cell lines were obtained from the following sources: OCI-LY-19, OCI-LY-10 and HBL-1 from Dr. C. Henry; SUDHL-4, SUDHL-6 and U2932 from Dr. L. Mizrachi-Bernal, Emory University, Atlanta, GA. HEK 293T cells were obtained from American Type Culture Collection.
Bajpai R., Sharma A., Achreja A., Edgar C.L., Wei C., Siddiqa A.A., Gupta V.A., Matulis S.M., McBrayer S.K., Mittal A., Rupji M., Barwick B.G., Lonial S., Nooka A.K., Boise L.H., Nagrath D, & Shanmugam M. (2020). Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma. Nature Communications, 11, 1228.
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5

Isolation and Characterization of Myeloma and NK Cells

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Following cell lines KMS11, H929, RPMI8226, U266, K562 were purchased from ATCC (Manassas, VA, USA). OCI-MY5, OPM2, ARK, RPMI8226/R5 (MM-resistant cell line) were a kind gift from Professor Zhan (Department of Internal Medicine, University of Iowa, Iowa, USA). K562 was maintained in Iscove's Modified Dubecco's Medium (Gibco), supplemented with 10% fetal bovine-serum (Gibco) and 1% penicillin streptomycin–glutamine (Gibco). Other cells were grown in suspension in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine-serum (15% for U266) and 1% penicillin streptomycin–glutamine. All cells were incubated at 37°C in 5% carbon dioxide air atmosphere. CD138+ myeloma cells and CD56+/CD3− NK cells were isolated from bone marrow and peripheral blood using magnetic bead selection (Miltenyi Biotech, Auburn, CA). Informed consent was obtained from each patient. All selected cells were more than 95% pure.
Yang G., Gao M., Zhang Y., Kong Y., Gao L., Tao Y., Han Y., Wu H., Meng X., Xu H., Zhan F., Wu X, & Shi J. (2015). Carfilzomib enhances natural killer cell-mediated lysis of myeloma linked with decreasing expression of HLA class I. Oncotarget, 6(29), 26982-26994.
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6

Cell Line Culture Protocols

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All the cell lines (A549, HTC116, MiaPaCa-2, SKOV-3, SW480, PC-3, SK-BR-3, K562, MDA-MB-231, A498, HT-29, Jurkat, KMS-11, RPMI-8226, MM.1S, REH, U266, and K562) were purchased from ATCC. As recommended by the manufacturer, the cells were cultured with Dulbecco’s Modified Eagle’s Medium, minimum essential media, or F-12K medium supplemented with 10% fetal bovine serum, penicillin to 100 units/mL, and streptomycin to 100 µg/mL. The cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 and 95% air.
Wang Y., Wallach J., Duane S., Wang Y., Wu J., Wang J., Adejare A, & Ma H. (2017). Developing selective histone deacetylases (HDACs) inhibitors through ebselen and analogs. Drug Design, Development and Therapy, 11, 1369-1382.
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7

Culturing Human Cancer Cell Lines

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Human multiple myeloma cells (MM.1S and KMS11, ATCC) were cultured in RPMI media (Thermo Fisher Scientific) and were supplemented with 10% fetal bovine serum (FBS, VWR), 1% penicillin/streptomycin (Thermo Fisher Scientific), and 1% glutamine (Thermo Fisher Scientific). Human lung adenocarcinoma cells (A549, ATCC) were cultured in RPMI media, which was supplemented with 10% FBS and 1% penicillin/streptomycin. Cell lines were authenticated by short tandem repeat DNA profiling and were confirmed to be mycoplasma negative, using the MycoAlert Mycoplasma Testing Kit (Lonza). All cells were housed in 5% CO2 humidified atmosphere at 37 °C.
Nguyen H.V., Detappe A., Harvey P., Gallagher N., Mathieu C., Agius M.P., Zavidij O., Wang W., Jiang Y., Rajca A., Jasanoff A., Ghobrial I.M., Ghoroghchian P.P, & Johnson J.A. (2020). Pro-organic radical contrast agents (“pro-ORCAs”) for real-time MRI of pro-drug activation in biological systems. Polymer chemistry, 11(29), 4768-4779.
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8

Inhibition of Aminopyrimidine Kinase in Multiple Myeloma

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The aminopyrimidine kinase inhibitor JP11646 and other members of its family of inhibitors (Table 1), LGB321 (Novartis, Berkeley, CA) and AZD1208 (Astra Zeneca, London, UK) were synthesized and obtained from Jasco Pharmaceuticals (Woburn, MA) and were used as 10 mM stocks in DMSO. The MM cell lines MM1.S (gift from Dr. Stephen Rosen, Chicago, IL), RPMI8226, U266 and KMS11 (ATCC, Manassas, VA), were maintained in RPMI 1640 media (Mediatech Inc, Manassas, VA) containing 10% FBS (Hyclone Inc., Logan, UT), 1000 U/ml penicillin/streptomycin, 4 mM L-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate. Recombinant IL-6 was purchased from R&D Systems (Minneapolis, MN).
Nair J.R., Caserta J., Belko K., Howell T., Fetterley G., Baldino C, & Lee K.P. (2016). Novel inhibition of PIM2 kinase has significant anti-tumor efficacy in multiple myeloma. Leukemia, 31(8), 1715-1726.
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9

Multiple myeloma cell lines characterization

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Multiple myeloma cell lines (HLA-A24+ KMS11; HLA-A24− OPM1) were obtained from the ATCC (Manassas, VA). A T2 cell line transduced with HLA-A*2401 cDNA (T2-A24 cells) was provided by Dr. Y. Miyahara at Mie University School of Medicine (Mie, Japan) and Dr. E. Jaffee at the Johns Hopkins University School of Medicine (Baltimore, MD). LNCaP (HLA-A24+; XBP1un− XBP1sp− CS1−) and THP-1 (HLA-A24+; CD138−) obtained from ATCC were used as MHC HLA-A24 matched antigen-specific negative control cell lines. All cell lines were authenticated by STR profiling, tested for mycoplasma contamination and cultured in RPMI-1640 or DMEM medium (Gibco-Life Technologies, Rockville, MD) supplemented with 10% fetal calf serum (FCS; BioWhittaker, Walkersville, MD), 100 IU/ml penicillin, and 100 μg/ml streptomycin (Gibco-Life Technologies).
Bae J., Hideshima T., Zhang G.L., Zhou J., Keskin D.B., Munshi N.C, & Anderson K.C. (2017). Identification and characterization of HLA-A24 specific XBP1, CD138 (Syndecan-1), and CS1 (SLAMF7) peptides inducing antigens-specific memory cytotoxic T lymphocytes targeting multiple myeloma. Leukemia, 32(3), 752-764.
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10

Cell Line Culture Protocols

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MM cell lines (MM.1S, NCI-H929, RPMI 8226, LP1, U266, L363, and KMS11) were purchased from ATCC. The cell lines were cultured in RPMI 1640 supplemented with 10% or 2% (prior to infection) fetal bovine serum (FBS) (catalog no. 019K8420, Sigma), 100 IU/mL penicillin, and 100 μg/mL streptomycin. African green monkey kidney epithelial Vero cells were cultured in DMEM supplemented with 10% or 2% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin.
Ghose J., Dona A., Murtadha M., Gunes E.G., Caserta E., Yoo J.Y., Russell L., Jaime-Ramirez A.C., Barwick B.G., Gupta V.A., Sanchez J.F., Sborov D.W., Rosen S.T., Krishnan A., Boise L.H., Kaur B., Hofmeister C.C, & Pichiorri F. (2021). Oncolytic herpes simplex virus infects myeloma cells in vitro and in vivo. Molecular Therapy Oncolytics, 20, 519-531.
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