Log In Sign up
The largest database of trusted experimental protocols

Human tgf beta 1 elisa kit

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom, United States

The Human TGF beta 1 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of TGF beta 1 levels in human cell culture supernatants and serum samples.

Automatically generated - may contain errors

Lab products found in correlation

Bicinchoninic acid assay, by Merck Group (2 mentions) Belysa immunoassay curve fitting software, by Merck Group (2 mentions) 96 plate reader, by Enzo Life Sciences (2 mentions) Softmax pro 7, by Molecular Devices (2 mentions) Spectra max plus 384 microplate spectrophotometer, by Molecular Devices (2 mentions) Quantikine hs elisa human il10, by RnD Systems (2 mentions) Human interleukin 35 il35 elisa kit, by Nordic Biosite (2 mentions) Easysep human t cell isolation kit, by STEMCELL (1 mentions) T cell transact, by Miltenyi Biotec (1 mentions) Legend max human ifn γ elisa kit, by BioLegend (1 mentions) Hydroxyproline colorimetric assay kit, by Abcam (1 mentions) Lxsahm 03, by R&D Systems (1 mentions) Human il 6 elisa kit, by Abcam (1 mentions) Hydroxyproline assay kit, by Merck Group (1 mentions) Human elastin elisa kit, by Abcam (1 mentions)

Spelling variants of this product

ELISAs (10 citations) TGF beta 1 (6 citations) TGFß1 (3 citations)

9 protocols using human tgf beta 1 elisa kit

1

Quantifying TGF-β1 and IFN-γ in Humanized Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total TGF-β1 level in the plasma from humanized mice was quantified by TGF beta 1 Human ELISA Kit (Abcam) according to manufacturer’s instructions.
Tumor-infiltrating T cells in humanized mice were isolated using EasySep Human T Cell Isolation kit (Stemcell Technologies). Purified T cells (5 × 105 cells/mL) were seeded into a 96-well plate and stimulated with T Cell TransAct (Miltenyi Biotec) for 24 h. After incubation, supernatant was collected and centrifuged once to remove cell debris. Human IFN-γ level in the cell-free supernatant was determined by LEGEND MAX Human IFN-γ ELISA Kit (BioLegend).
Liu W.N., Fong S.Y., Tan W.W., Tan S.Y., Liu M., Cheng J.Y., Lim S., Suteja L., Huang E.K., Chan J.K., Iyer N.G., Yeong J.P., Lim D.W, & Chen Q. (2020). Establishment and Characterization of Humanized Mouse NPC-PDX Model for Testing Immunotherapy. Cancers, 12(4), 1025.
+ Open protocol
+ Expand
2

TGFβ-1 Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
TGFβ−1 levels were measured by enzyme-linked immunoassay using the TGF beta 1 Human ELISA Kit (ab100647, Abcam). Levels were measured in 100 μl of cell culture media, in duplicate, according to the manufacturer’s instructions. Four representative individuals from each species were assayed on one 96-well plate. TGFβ−1 levels were quantified relative to a standard curve using 4- and 5-parameter logistic models implemented in the drc package in R. Final TGFβ−1 release is reported as four measurements: A (A-A), B (B-A), C (C-B) and D (D-B).
Ward M.C, & Gilad Y. (2019). A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees. eLife, 8, e42374.
+ Open protocol
+ Expand
3

Measuring Shh, TGFβ1, and Hydroxyproline

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of human Shh and TGFβ1 in AECII supernatant was measured by a commercially available ELISA kit: human Sonic Hedgehog ELISA kit (Abcam, ab100639) and human TGF beta 1 ELISA kit (Abcam, ab100647) according to the manufacturer’s instructions. The concentration of hydroxyproline was measured using Hydroxyproline Assay Kit (Colorimetric) (Abcam, ab222941) according to the manufacturer’s protocol.
Yang X., Sun W., Jing X., Zhang Q., Huang H, & Xu Z. (2022). C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis. Respiratory Research, 23, 86.
+ Open protocol
+ Expand
4

Multiplex Immunoassay for Fibroblast Secretome

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiplex immunoassays (LXSAHM-03, R&D Systems, Mineapolis, MN, USA) were used to investigate fibroblast expression levels of interleukin-6 (IL-6), interleukin-1 receptor antagonist (IL-1RA), and platelet-derived growth factor alpha (PDGF-α) in cell lysates following the manufacturer’s instructions. The Luminex MAGPIX system with xPONENT 4.3 software (Luminex Corporation, Austin, TX, USA. A DiaSorin Company) instrument was used for analyses of the multiplex immunoassays (using Belysa ® Immunoassay Curve Fitting Software (Merck KGaA, Darmstadt, Germany)). The ELISA assay (Human TGF beta 1 Elisa kit, ab108912, Abcam, Cambridge, UK) was further used for determining levels of transforming growth factor beta 1 (TGF-β1). Absorbance at 450 nm was measured using a 96 Plate Reader (Enzo Life Sciences, Inc., East Farmingdale, NY, USA) and additional background subtraction was done at 570 nm (analysis with Byonoy Software, Byonoy GmbH). Total protein quantification was done with a bicinchoninic acid assay (Sigma Aldrich, Merck KGaA, Darmstadt, Germany) and these data were used for normalization of the data.
Radstake W.E., Gautam K., Miranda S., Vermeesen R., Tabury K., Rehnberg E., Buset J., Janssen A., Leysen L., Neefs M., Verslegers M., Claesen J., van Goethem M.J., Weber U., Fournier C., Parisi A., Brandenburg S., Durante M., Baselet B, & Baatout S. (2023). The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the In Vitro Wound Healing Process. Cells, 12(2), 246.
+ Open protocol
+ Expand
5

Treg-specific Cytokine Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Quantikine HS ELISA Human IL10 (RnD Systems, cat. HS100C), Human TGF beta 1 ELISA kit (Abcam, cat. ab100647) and Human Interleukin 35 (IL35) ELISA Kit (Nordic BioSite, cat. EKX-6FHVKH-96) were used to measure the amount of Treg-specific cytokines in Treg suppression assay supernatant, according to instructions from the manufacturers. For IL35, samples were diluted 1:1 in sample dilution buffer, for IL10 and TGF- β , samples were handled according to manufacturer’s protocols. Absorbance was read at A450 nm (IL35 and TGF- β ), and A490 nm (IL10) using the SpectraMax plus 384 Microplate Spectrophotometer and SoftMax Pro 7.1 software (Molecular Devices).
Sjøgren T., Islam S., Filippov I., Jebrzycka A., Sulen A., Breivik L.E., Hellesen A., Jørgensen A.P., Lima K., Tserel L., Kisand K., Peterson P., Ranki A., Husebye E.S., Oftedal B.E, & Wolff A.S. (2024). Single cell characterization of blood and expanded regulatory T cells in autoimmune polyendocrine syndrome type 1. iScience, 27(4), 109610.
+ Open protocol
+ Expand
6

Fibroblast Cytokine and Growth Factor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibroblast expression of pro-inflammatory cytokines and growth factors and were quantified in lysates (see “Extracellular matrix protein expression” for a description of how lysates were collected) with Multiplex immunoassay (Interleukin-6 (IL-6), Interleukin-1 receptor agonist (IL-1RA), Luminex Discovery Assay, LXSAHM-03, R&D systems) and ELISA assays (transforming growth factor-beta (TGF-β1), Human TGF beta 1 ELISA kit, ab108912, Abcam) following manufactures instructions. Analyses was performed on Luminex (MAGPIX system with xPONENT 4.3 software (Luminex Corporation. A DiaSorin Company)) instrument for the Multiplex immune assay (analysis performed with Belysa® Immunoassay Curve Fitting Software (Merck, KGaA) and on the absorbance 96 Plate Reader (Enzo Life Sciences, Inc) measured at 450 nm and background subtraction at 570 nm (analysis performed with Byonoy software (Byonoy GmbH). Total protein quantification was done with bicinchoninic acid assay (Sigma-Aldrich), and these data were used for normalization of the data.
Radstake W.E., Gautam K., Miranda S., Van Rompay C., Vermeesen R., Tabury K., Verslegers M., Dowson A., Gorissen J., van Loon J.J., Savage N.D., Baatout S, & Baselet B. (2023). Gravitational effects on fibroblasts’ function in relation to wound healing. NPJ Microgravity, 9, 48.
+ Open protocol
+ Expand
7

Quantification of Treg-Specific Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Treg-specific cytokines were measured in supernatant from the suppression assay at the end of culturing using Quantikine HS ELISA Human IL-10 (RnD Systems, cat. HS100C), Human TGF beta 1 ELISA Kit (Abcam, cat. AB100647), and Human Interleukin 35 (IL-35) ELISA Kit (Nordic BioSite, cat. EKX-6FHVKH-96) according to instructions from the manufacturers. Absorbance was read at A450 nm (IL-35 and TGF-β), and A490 nm (IL-10) using a SpectraMax plus 384 Microplate Spectrophotometer and the SoftMax Pro 7.1 software (Molecular Devices).
Sjøgren T., Bjune J.I., Husebye E.S., Oftedal B.E, & Wolff A.S. (2023). Regulatory T cells in autoimmune primary adrenal insufficiency. Clinical and Experimental Immunology, 215(1), 47-57.
+ Open protocol
+ Expand
8

Quantifying TGF-β1 and IL-6 Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The production of TGF-β1 and IL-6 was assessed using commercial Human TGF beta 1 ELISA Kit (Abcam) and Human IL-6 ELISA Kit (Abcam), following the protocols, respectively.
Li Z., Chen Z., Li S., Qian X., Zhang L., Long G., Xie J., Huang X., Zheng Z., Pan W., Li H, & Zhang D. (2023). Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA. Cell Death Discovery, 9, 161.
+ Open protocol
+ Expand
9

Quantifying Skin Extracellular Matrix Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Detection of circulating chondroitin sulfate and hydroxyproline was evaluated in serum using CS ELISA Kit (abx350001—Abbexa, Sugar Land, TX, USA) and hydroxyproline assay kit (MAK008—Sigma-Aldrich, Saint-Quentin-Fallavier, France), respectively. In HDFs, elastin, hyaluronan and TGF-β levels were evaluated in cell culture supernatant using Human Elastin ELISA Kit (ab239433—Abcam, Paris, France), Hyaluronan Quantikine ELISA Kit (DHYAL0—Bio-Techne, Minneapolis, MN, USA) and Human TGF beta 1 ELISA Kit (ab100647—Abcam, Paris, France), respectively, according to the manufacturer’s recommendations. Measurements were performed in quadruplicates for each sample condition of the ten volunteers.
Wauquier F., Boutin-Wittrant L., Bouvret E., Le Faouder J., Roux V., Macian N., Pickering G, & Wittrant Y. (2022). Benefits of Circulating Human Metabolites from Fish Cartilage Hydrolysate on Primary Human Dermal Fibroblasts, an Ex Vivo Clinical Investigation for Skin Health Applications. Nutrients, 14(23), 5027.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!