The ECL assays for FRA, MSLN, and MPF used in the present analyses have been described previously.43 (link),44 Samples (serum, urine) from healthy or diseased subjects and standards were added to wells of 96-well plates previously coated with marker specific capture monoclonal antibody (MAb) and incubated at room temperature for two hours. The ruthenium labeled detection MAbs were diluted in assay buffer, added to washed plates, and incubated for an additional two hours at room temperature. Plates were washed, read buffer added, and signals measured using an MSD DISCOVERY WORKBENCH® (Mesoscale Discovery, Gaithersburg, MD). Optimal sample dilutions were: FRA (80-fold dilution of urine and a 20-fold dilution of serum), MSLN (60-fold dilution of urine and an 80-fold dilution of serum), and MPF (4-fold dilution of urine and a 20-fold dilution of serum).
Msd discovery workbench
Manufactured by Mesoscale
The MSD Discovery Workbench is a laboratory equipment product offered by Mesoscale. It is designed for performing various analytical tasks in a controlled environment. The core function of the MSD Discovery Workbench is to provide a versatile and customizable workspace for conducting experiments and analyses.
Automatically generated - may contain errors
3 protocols using msd discovery workbench
1
ECL Assays for FRA, MSLN, and MPF
2
Quantification of Amyloid-Beta Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Incubation medium from vehicle-only (control) and pharmacologically treated 3-week-old tissue cultures was collected, frozen immediately with dry ice, and stored at −80°C (vehicle, n = 6 wells with 36 tissue cultures; BACE inhibitor C3, n = 3 wells with 18 tissue cultures; Begacestat, n = 3 wells with 18 tissue cultures). For the detection of Aβ, a V-PLEX Aβ Peptide Panel 1 Kit (MesoScale Discovery, catalog #K15199E, RRID:AB_2827747 ) was used with a ruthenylated anti-Αβ antibody (4G8 clone). The Aβ peptide concentration was determined using the MSD Discovery Workbench software (MesoScale Discovery).
3
Quantifying SARS-CoV-2 Antibodies and Neutralization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SARS-CoV-2 N-protein concentrations in sera were quantified using the S-PLEX SARS-CoV-2 N Kit (Meso Scale Diagnostics, Rockville, MD, USA) according to the manufacturer’s protocol. Similarly, the concentrations of α-SARS-CoV-2 IgG antibodies and their neutralization capacity in sera were quantified using the SARS-CoV-2 Plate 7 (Meso Scale Diagnostics). Immune assays from Meso Scale were acquired via the MESO QuickPlex SQ 120 imager (Meso Scale Diagnostics) and analyzed with the MSD Discovery Workbench (Meso Scale Diagnostics).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!