Anti cd166 alcam mab mog 07

TaqMan® MicroRNA assays for miRNA detection: Hsa-miR-214 ID 002306, Hsa-miR-148b ID000471, Hsa-miR-223 ID 002295 U6 snRNA ID001973, (all from Applied Biosystems, Foster City, USA). TaqMan® Gene expression assays for S100b: ID Mm00485897_m1. Primary antibodies: anti-ITGA5 pAb RM10 kindly provided by G. Tarone laboratory (Molecular Biotechnology Center, University of Torino), anti-CD166/ALCAM mAb MOG/07 (Novocastra Laboratories), anti-E-cadherin mAb #610,182 (BD Transduction Laboratories, Franklin Lakes, USA), anti-N-cadherin pAb ab18203 and anti-αSMA pAb ab15734 (Abcam, Cambridge, United Kingdom), anti-TFAP2C mAb 6E4/4 mAb, H-77 pAb, anti-GAPDH pAb V-18, anti-ACTIN I-19 pAb, anti-Hsp90 mAb F-8 (all from Santa Cruz Biotechnology). Secondary antibodies: HRP-conjugated goat anti-mouse IgG, goat anti-rabbit IgG (all from Santa Cruz Biotechnology, Santa Cruz, CA), goat anti-rabbit IgG Alexa-Fluor-488 (Molecular Probes, Invitrogen Life Technologies, Carlsbad, USA). All antibodies were used at the producer’s suggested concentration.

miR precursors: Pre-miR™ miRNA Precursor Negative Control #1, Pre-miR™ miRNA Precursor hsa-miR-148b (PM10264) (Applied Biosystems). TaqMan® MicroRNA assays for miRNA detection: Hsa-miR-148b-3p ID 000471, U6 snRNA ID001973, U44 snRNA ID001904 (Applied Biosystems). Quantitect Primer Assay: 218300Axl ID 33000 (Qiagen). Qiagen miScript-SYBR Green PCR Kit and miScript Primer Assay: hsa-let-7g ID 1 (Qiagen). Primary antibodies: anti-Cleaved Caspase-3 (Asp175) #9661 (Cell Signaling Technology), anti-Ki67 ab15580 (Abcam), anti-ITGA5 pAb RM10 kindly provided by G. Tarone laboratory (Molecular Biotechnology Center, University of Torino), anti-CD166/ALCAM mAb MOG/07 (Novocastra Laboratories), anti-GAPDH pAb V-18, anti-ACTIN I-19 pAb (all form Santa Cruz Biotechnology), anti-AXL (R&D Systems). Secondary antibodies: HRP-conjugated goat anti-mouse IgG, goat anti-rabbit IgG (Santa Cruz Biotechnology).