Succinic acid assay kit
The Succinic Acid Assay Kit is a laboratory equipment used to quantify the concentration of succinic acid in a sample. It provides a rapid and accurate method to measure succinic acid levels.
3 protocols using succinic acid assay kit
Measuring Serum Succinate Levels in Rats
Succinic Acid Production in Methanotrophic Bacteria
Example 3
Positive transformants of M. capsulatus containing the malate dehydrogenase (mdh; SEQ ID 1; pSB107), pyruvate carboxylase (pyc; SEQ ID 2, pSB108), phospho enol pyruvate carboxylase (ppc; SEQ ID 3, pSB109) genes were verified by PCR. These were inoculated into 5 ml of liquid NMS media taken in 30 ml culture tubes and sealed with suba seals. 15 ml of Methane mixture (95% CH4; 5% CO2) was introduced into the culture tube using a syringe. The tubes were incubated at 45° C. at 250 rpm agitation. Once the culture OD reached 1, the cultures was centrifuged and the supernatant samples were taken and assayed for succinic acid. The organic acid concentrations were measured using Succinic Acid assay kit (Megazyme International; K-SUCC) according to manufacturer's protocol (
Some of the recombinant strains had higher levels of succinic acid compared to the control strain. Recombinant strain with SEQ ID 1 was >15% improved when compared to the native strain. Recombinant strain with SEQ ID 2 was >50% improved when compared to the native strain.
Metabolite Dynamics in Cell Cultures
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