Anti mouse cd3e fitc anti mouse mhc 2 pe

The levels of CD4⁺ and CD8⁺ T cell subsets and the levels of MHC-I and MHC-II molecules in the splenocytes of mice from the four test groups, pVAX-EF-1α, pVAXI, PBS, and blank, were determined using flow cytometry as previously described [26 (link)]. Splenocyte suspensions (1 × 10⁶ cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE, anti-mouse CD3e-FITC + anti-mouse CD4-PE, anti-mouse CD3e-FITC + anti-mouse MHC-I-PE or anti-mouse CD3e-FITC + anti-mouse MHC-II-PE (eBioscience, San Diego, CA, USA) for 30 min at 4 °C in the dark. Cell population analyses were conducted with a FACScan flow cytometer with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). Lymphocyte specific gating was set according to the forward and side scatter profiles. The percentages of CD4⁺ and CD8⁺ T lymphocytes or, MHC-I and MHC-II molecules in mouse splenocytes were determined as previously described [27 (link)].

The percentages of T cells subsets CD4⁺ and CD8⁺, beside MHC-I and MHC-II molecules in the splenocytes of mice in the test groups, rTgEF-1α, adjuvant, PBS, and blank, were determined using flow cytometry technique as described previously (Sasai et al., 2000 (link)). Splenocytes suspensions (1 × 10⁶ cells/ml) were dually stained with anti-mouse CD3e-FITC+anti-mouse CD8-PE, anti-mouse CD3e-FITC+anti- mouse CD4-PE, anti-mouse CD3e-FITC+anti-mouse MHC-I-PE or anti-mouse CD3e-FITC+anti- mouse MHC-II-PE (eBioscience) for 30min at 4°C in the dark. Cell population analysis was conducted by FACScan flow cytometer with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). A lymphocyte specific gating was set according to forward and side scatters profiles. The percentages of CD4⁺ and CD8⁺ T lymphocytes, MHC-I and MHC-II molecules in mice splenocytes were determined as described by Song et al. (2010) (link).

The percentages of T cells subsets CD4⁺ and CD8⁺, beside MHC-I and MHC-II molecules in the spleenocytes of mice in the test groups, pTgDPA and pVAX1, PBS and blank, were analyzed using the flow cytometry technique as described by [22 (link)].
Splenocytes suspensions (1 × 10⁶ cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE, anti-mouse CD3e-FITC + anti-mouse CD4-PE, anti-mouse CD3e-FITC + anti-mouse MHC-I-PE or anti-mouse CD3e-FITC + anti- mouse MHC-II-PE (eBioscience) for 30 min at room temperature in the dark. Cell population analysis was conducted by FACScan flow cytometry with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). A lymphocyte specific gating was set according to forward and side scatters profiles. The percentages of CD4⁺ and CD8⁺ T lymphocytes, MHC-I and MHC-II molecules in mice spleenocytes were determined as described by [23 (link)].