The following antibodies were used for western blot analysis: anti-PD-L1 (cat.no. NBP1-76769; NOVUS), anti-β-actin (cat.no. ab8224; Abcam), anti-NOX4 (cat.no. ab109225; Abcam), anti-ZNF24 (cat.no. NBP1-82866; NOVUS), anti-UBE2I (cat.no. ab75854; Abcam), and anti-SUMO-1 (cat.no. ab32058; Abcam). Antibodies used for immunohistochemistry analysis were the following: anti-PD-L1 (cat.no. NBP1-76769; NOVUS), anti-NOX4 (cat.no. ab109225; Abcam), anti-ZNF24 (cat.no. NBP1-82866; NOVUS), and anti-CD8 (cat.no. ab237709; Abcam). Anti-UBE2I (cat.no. ab75854; Abcam), anti-SUMO-1 (cat.no. ab32058; Abcam), and anti-ZNF24 (cat.no. A303-091A; Thermo Fisher Scientific) were used for Immunoprecipitation (IP). Oleic acid, palmitic acid, Oil Red O, and MitoTEMPO were purchased from Sigma-Aldrich (St. Louis, MO). Reactive oxygen species (ROS) assay, Nacetyl-cysteine (NAC), JC-1-Mitochondrial Membrane Potential Assay Kit, and LDH Cytotoxicity Assay Kit were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). Alanine aminotransferase (ALT) and aspartate transaminase (AST) assay kit were acquired from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China).
Anti znf24
Manufactured by Abcam
Anti-ZNF24 is a recombinant antibody that recognizes the ZNF24 protein. ZNF24 is a zinc finger protein that functions as a transcriptional regulator. This antibody can be used for applications such as Western blotting and immunohistochemistry to detect ZNF24 expression.
Automatically generated - may contain errors
Lab products found in correlation
Anti runx1, by Abcam (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Merck Group (1 mentions)
Anti vegfa, by Abcam (1 mentions)
Anti h3k27me3, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti nox4, by Abcam (1 mentions)
Anti nox4, by Novus Biologicals (1 mentions)
Anti cd8, by Novus Biologicals (1 mentions)
Anti sumo1, by Abcam (1 mentions)
Anti β actin, by Novus Biologicals (1 mentions)
Reactive oxygen species assay, by Beyotime (1 mentions)
Mitotempo, by Merck Group (1 mentions)
Oil red o, by Merck Group (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Alanine aminotransferase alt, by Nanjing Jiancheng (1 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Beyotime (1 mentions)
N acetylcysteine nac, by Beyotime (1 mentions)
2 protocols using anti znf24
1
Antibodies and Reagents for Proteomics
2
Protein Extraction and Western Blotting Protocol
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Total proteins of the cells were extracted by Golden lysis buffer (Tris-HCl [pH 8.0], 400 mM NaCl, 5 mM EDTA, 1 mM EGTA, 1 mM Na pyrophosphate, 1% Triton X-100, 10% glycerol), with a supplement of protease inhibitors (Roche, Indianapolis, IN, USA). The concentration of total protein was measured with a bicinchoninic acid (BCA) kit (Pierce, Rockford, IL, USA). 20 mg of protein was loaded onto 10% SDS-PAGE gel and was transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Membranes were then incubated with primary and secondary antibodies. Protein signals were detected via the enhanced chemiluminescence (ECL) method. The primary antibodies used in this study include anti-VEGFA (1:1,000, Abcam), anti-ZNF24 (1:1,000, Abcam), anti-GAPDH (1:2,000, Abcam), anti-Runx1 (1:1,000, Abcam), and anti-H3K27me3 (1:1,000, Abcam). All primary antibodies were incubated overnight at 4°C. The horseradish peroxidase (HRP)-conjugated secondary antibody (1:5,000, Sigma-Aldrich) was incubated for 2 h at room temperature.
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