At the end of the experiments, lung tissues were incubated with anti-ICAM-1 murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology), anti-P-selectin murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology), anti-PAR murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology) and anti-nitrotyrosine rabbit polyclonal antibody (1:200 in PBS, v/v, Millipore), as previously described [72 (link),88 (link),103 (link),104 (link),105 (link),106 (link),107 (link),108 (link)]. Immunohistochemical images were collected using (Leica DM6, Milan, Italy) associated with an Imaging system (LasX Navigator, Milan, Italy). The digital images were opened in ImageJ, followed by deconvolution using the color deconvolution plug-in. When the IHC profiler plug-in is selected, it automatically plots a histogram profile of the deconvoluted DAB image, and a corresponding scoring log is displayed. The histogram profile corresponds to the positive pixel intensity value obtained from the computer program. All immunohistochemical analyses were carried out by two observers blinded to the treatment [72 (link),88 (link),103 (link),104 (link),105 (link),106 (link),107 (link),108 (link),109 (link)].
Anti icam 1 murine polyclonal antibody
Manufactured by Santa Cruz Biotechnology
Anti-ICAM-1 murine polyclonal antibody is a laboratory reagent produced by Santa Cruz Biotechnology. It is a purified polyclonal antibody derived from mice that specifically binds to the intercellular adhesion molecule-1 (ICAM-1) protein.
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2 protocols using anti icam 1 murine polyclonal antibody
1
Immunohistochemical Analysis of Lung Tissue
2
Lung Tissue Immunohistochemical Analysis
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At the end of experiments lung tissue were incubated with anti-ICAM-1 murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology), anti-P-selectin murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology), anti-PAR murine polyclonal antibody (1/100 in PBS, v/v, Santa Cruz Biotechnology) and anti-nitrotyrosine rabbit polyclonal antibody (1:200 in PBS, v/v, Millipore) as previously described [29-31, 42, 53, 57, 74-77] . Immunohistochemical images were collected using (Leica DM6, Milan, Italy) associated with an Imaging system (LasX Navigator, Milan, Italy). The digital images were opened in ImageJ, followed by deconvolution using the color deconvolution plug-in. When the IHC profiler plug-in is selected, it automatically plots a histogram profile of the deconvoluted DAB image, and a corresponding scoring log is displayed. The histogram profile corresponds to the positive pixel intensity value obtained from the computer program. All immunohistochemical analyses were carried out by two observers blinded to the treatment [29-31, 42, 74-78] .
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