HEK293T, HEK293FT and the human pancreatic cancer cell lines AsPC1, BxPC3, Capan1, Capan2, MIA PaCa2 and PANC1 were
obtained from ATCC (American Type Culture Collection) and maintained in a humidified 5% CO2 atmosphere at 37°C.
The mouse embryonic fibroblast (MEF) cells established from PRRX1 WT or KO mice was kindly provided by Kaori Ihida-Stansbury
(UPenn, Philadelphia, PA). The Capan1 cells were grown in DMEM (Corning) supplemented with 20% fetal bovine serum (FBS; SH3007103,
GE) and 100U/mL penicillin-streptomycin (15140122, Gibco, ThermoFisher) while AsPC1 and BxPC3 cells were cultured in RPMI1640
(Corning) supplemented with 10% FBS and 100U/mL penicillin-streptomycin. All other cell lines were grown in DMEM supplemented with
10% FBS and 100U/mL penicillin-streptomycin. During the course of this study all cell lines were tested for mycoplasma
contamination. Etoposide and the FOXM1 inhibitor, FDI6, were purchased from Selleck Chem (S1225) and Axon MedChem (2384)
respectively. Gemcitabine HCl solution was purchased from Hospira, inc (Lake Forest, IL, USA). A Gammacell 40 Cesium 137
Irradiation unit was used to deliver 8Gy.
obtained from ATCC (American Type Culture Collection) and maintained in a humidified 5% CO2 atmosphere at 37°C.
The mouse embryonic fibroblast (MEF) cells established from PRRX1 WT or KO mice was kindly provided by Kaori Ihida-Stansbury
(UPenn, Philadelphia, PA). The Capan1 cells were grown in DMEM (Corning) supplemented with 20% fetal bovine serum (FBS; SH3007103,
GE) and 100U/mL penicillin-streptomycin (15140122, Gibco, ThermoFisher) while AsPC1 and BxPC3 cells were cultured in RPMI1640
(Corning) supplemented with 10% FBS and 100U/mL penicillin-streptomycin. All other cell lines were grown in DMEM supplemented with
10% FBS and 100U/mL penicillin-streptomycin. During the course of this study all cell lines were tested for mycoplasma
contamination. Etoposide and the FOXM1 inhibitor, FDI6, were purchased from Selleck Chem (S1225) and Axon MedChem (2384)
respectively. Gemcitabine HCl solution was purchased from Hospira, inc (Lake Forest, IL, USA). A Gammacell 40 Cesium 137
Irradiation unit was used to deliver 8Gy.