Sample and matrix application followed the procedure outlined for above (MALDI TOF MS). The MALDI FT-ICR experiments were done using a solariX-XR 12 T FT-ICR mass spectrometer equipped with a dual electrospray ionization (ESI) and MALDI source (Bruker Daltonics, Bremen, Germany). The data set size was 1 MW (~0.5 s transient length), the detection was in magnitude mode with ramped excitation, and the m/z range was 200–3000. The MALDI source had the following parameters: the laser was set to 1 kHz at a laser power of ~30% (instrument specific setting), smartwalk with selective accumulation was enabled, and 500 laser shots per acquisition and 15 scans were summed. All data were acquired and processed with Data-Anaylsis 4.2 (Bruker Daltonics).
Maldi source
Manufactured by Bruker
Sourced in Germany
The MALDI source is a critical component of Bruker's mass spectrometry instruments. It is used to ionize and desorb analytes from a sample for subsequent mass analysis. The MALDI source employs a laser to generate ions from the sample, which are then detected and analyzed by the mass spectrometer.
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Lab products found in correlation
2 protocols using maldi source
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MALDI FT-ICR Mass Spectrometry Protocol
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MALDI Imaging of Metabolites in Tissue
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Before MALDI analysis, matrix coating (either 12 or 14 passes) was applied to the desiccated tissue sections by using an HTX M5 sprayer (HTX Technologies) with 10 mg mL−1N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC; Sigma), dissolved in 70:30 methanol:water. The spraying parameters were set as follows: 80 °C nozzle temperature, 0.1 mL min−1 flow rate, 1000 mm min−1 velocity, 2 mm track spacing, 10 psi pressure, 3 L min−1 gas flow rate, and 10 s drying time for each pass.
MALDI samples were analyzed by using a timsTOF fleX with a MALDI source (Bruker Daltonics). All MALDI images were collected in negative polarity in MS1 mode at 50 μm pixel size. The laser was set as a single focused beam with a laser power of 85%, a laser frequency of 10 kHz, and 200 shots per pixel. The mass range was 50-400 m/z. Mass calibrations were performed by using 1 mM sodium formate solution. AMP, stearate, and palmitate were analyzed from sections with 12 layers of matrix coating, while NAA was analyzed in sections with 14 layers applied.
MALDI samples were analyzed by using a timsTOF fleX with a MALDI source (Bruker Daltonics). All MALDI images were collected in negative polarity in MS1 mode at 50 μm pixel size. The laser was set as a single focused beam with a laser power of 85%, a laser frequency of 10 kHz, and 200 shots per pixel. The mass range was 50-400 m/z. Mass calibrations were performed by using 1 mM sodium formate solution. AMP, stearate, and palmitate were analyzed from sections with 12 layers of matrix coating, while NAA was analyzed in sections with 14 layers applied.
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