Silicon dioxide (SiO2) (granular, ≥ 99.9%), silver nitrate (AgNO3, crystals, ≥ 99.9) and culture media were purchased from Sigma–Aldrich Chemie, Steinheim, Germany. The Agricultural Research Center in Giza, Egypt, provided seeds of the faba bean cultivar (Giza 429) and the fungicide, Dithane M-45 (ethylene bis dithiocarbamate at 80%, 16 percent manganese, and just 2% zinc). Escherichia coli D8 (AC: MF062579) and B. cinerea (AC: KP151604) were provided by the Laboratory of Microbiology, Faculty of Science, Damietta University, Egypt. Botrytis cinerea was cultivated on faba bean dextrose agar (FDA) and incubated for 7 days at 25 °C.
Silicon dioxide sio2
Manufactured by Merck Group
Sourced in Germany, United States
Silicon dioxide (SiO2) is a chemical compound that consists of one silicon atom and two oxygen atoms. It is a common and naturally occurring material that is widely used in various industries, including laboratory equipment manufacturing. As a lab equipment product, silicon dioxide serves as a core component in the fabrication of various laboratory devices and instruments.
Automatically generated - may contain errors
Lab products found in correlation
Silver nitrate agno3, by Merck Group (1 mentions)
Propylene oxide, by Merck Group (1 mentions)
Aluminum chloride hexahydrate, by Merck Group (1 mentions)
Copper 2 nitrate trihydrate, by Merck Group (1 mentions)
Polyethylene oxide, by Merck Group (1 mentions)
Ethanol, by RCI Labscan (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Degussa p25, by Evonik (1 mentions)
Metformin, by Beyotime (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Mrc 5, by Thermo Fisher Scientific (1 mentions)
Bish1475, by Sartorius (1 mentions)
Mrc 5, by Merck Group (1 mentions)
Rpmi medium 1640 basic, by Thermo Fisher Scientific (1 mentions)
Dmem 1640, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Merck Group (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Metformin, by Merck Group (1 mentions)
4 protocols using silicon dioxide sio2
1
Faba Bean Pathogenesis Assay
2
Synthesis and Characterization of Alumina-Supported Catalysts
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Polyethylene
oxide (PEO)
(99% purity), propylene oxide (PO) (≥99% purity), aluminum
chloride hexahydrate (AlCl3·6H2O) (99%
purity), copper (II) nitrate trihydrate (Cu(NO3)2·3H2O) (99% purity), and silicon dioxide (SiO2) (99.5% purity) were obtained from Sigma-Aldrich (St. Louis,
MO). γ-Aluminum oxide (99.97% purity) was obtained from Alfa
Aesar (Ward Hill, MA) and used as the commercial alumina support.
Ethanol (99.9% purity) was purchased from RCI Lab-Scan (Bangkok, Thailand).
Hydrogen (H2), carbon dioxide (CO2) Helium (He),
and argon (Ar) gases (99.999% of purity) were obtained from Thai Special
Gas (Pathumthani, Thailand).
oxide (PEO)
(99% purity), propylene oxide (PO) (≥99% purity), aluminum
chloride hexahydrate (AlCl3·6H2O) (99%
purity), copper (II) nitrate trihydrate (Cu(NO3)2·3H2O) (99% purity), and silicon dioxide (SiO2) (99.5% purity) were obtained from Sigma-Aldrich (St. Louis,
MO). γ-Aluminum oxide (99.97% purity) was obtained from Alfa
Aesar (Ward Hill, MA) and used as the commercial alumina support.
Ethanol (99.9% purity) was purchased from RCI Lab-Scan (Bangkok, Thailand).
Hydrogen (H2), carbon dioxide (CO2) Helium (He),
and argon (Ar) gases (99.999% of purity) were obtained from Thai Special
Gas (Pathumthani, Thailand).
3
Characterization of Engineered Nanomaterials
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Chemicals such as N-acetylcysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). UV light-responsive pure TiO2 NPs (Degussa P25; Evonik, Germany) were used as previously described12 (link),19 (link),32 (link). The crystal structure of P25 TiO2 was a mixture of 75% anatase and 25% rutile TiO2. The purity was at least 99.5% TiO2 and primary particle size was 21 nm ± 10 nm, with a specific surface area of 50 ± 15 m2/g. Carbon-containing TiO2 NPs [TiO2(C)] were prepared using a sol–gel method19 (link),33 (link). The powders were subjected to calcination at 200 °C, and named C200; the detailed preparation of C200 has been reported elsewhere19 (link),33 (link). By photoreduction process using H2PtCl6 and TiO2 NPs as a platinum precursor and a pristine photocatalyst, respectively, platinum-containing TiO2 NPs [TiO2(Pt)] were prepared and characterized following previously described methods13 (link),15 (link). The NPs of zinc oxide (ZnO; < 40 nm)9 (link) single-walled carbon nanotubes (CNTs), and silicon dioxide (SiO2; 10–20 nm) were purchased from Sigma–Aldrich. Nanodiamond (ND) NPs, with average sizes of 5 and 100 nm9 (link),34 (link),35 (link), were purchased from Kay Diamond Products (Boca Raton, FL, USA).
4
Investigating Metformin's Modulation of Cell Responses to Silica Nanoparticles
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The lung epithelial cells (A549 and HBE), lung fibroblast (MRC-5), and the human monocytic cell (THP-1) were commercially obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 and THP-1 cells were maintained in RPMI Medium 1640 basic (1640, Life Technologies/Gibco, Grand Island, NY, USA), the HBE cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, 1640, Life Technologies/Gibco, Grand Island, NY, USA), and the MRC-5 cells were maintained in Minimum Essential Medium (MEM, Life Technologies/Gibco, Grand Island, NY, USA). All of the culture media were containing 10% fetal bovine serum (BISH1475, Biological Industries) and antibiotics (penicillin and streptomycin, Life Technologies/Gibco, Gaithersburg, MD). Cells were cultured at 37 °C in a 5% CO2 atmosphere.
PMA (Sigma–Aldrich) was used to treat THP-1 cells into macrophages. For all the experiments analysis, epithelial cells and THP-1 were treated with 200 or 150 μg/ml Silicon dioxide (SiO2) (Sigma-Aldrich, St. Louis, MO, USA) together with various concentrations (2, 5, 10 mM) of metformin (Beyotime Institute of Biotechnology, Shanghai, China); MRC-5 cells were treated with 5 ng/ml TGF-β1 (Sigma-Aldrich) together with metformin.
PMA (Sigma–Aldrich) was used to treat THP-1 cells into macrophages. For all the experiments analysis, epithelial cells and THP-1 were treated with 200 or 150 μg/ml Silicon dioxide (SiO2) (Sigma-Aldrich, St. Louis, MO, USA) together with various concentrations (2, 5, 10 mM) of metformin (Beyotime Institute of Biotechnology, Shanghai, China); MRC-5 cells were treated with 5 ng/ml TGF-β1 (Sigma-Aldrich) together with metformin.
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