Fresh PBMCs were stained with anti-CD3-APC-Cy7, CD8-APC, CD45RA-PE-Cy5, CCR7-PE-Cy7, PD-1-Pacific Blue, IL-7Rα-FITC, CD27-PE, CD28-PE, CTLA4-PE, CX3CR1-PE antibodies or isotype control (all from BioLegend, San Diego, CA). PBMCs that had been stimulated with PMA/ionomycin were stained with anti-CD3-APC-Cy7, CD8-APC, CD45RAPE-Cy5 and CCR7-PE-Cy7 antibodies followed by fixation, permeabilization (Cytofix/Cytoperm Kit, BD Biosciences) and staining with anti-IFNγ-PE, TNF-α-FITC or IL-13-PE antibodies (BioLegend).14 (link) Stained cells were analyzed on an LSRII® flow cytometer (BD Biosciences). Collected data were analyzed using FlowJo software (Tree Star, Ashland, OR).
Pd 1 pacific blue
Manufactured by BioLegend
Sourced in United States
PD-1-Pacific Blue is a fluorescently-labeled antibody that binds to the Programmed Cell Death 1 (PD-1) receptor. PD-1 is an important immunoregulatory molecule expressed on T cells, B cells, and other immune cells. The Pacific Blue fluorescent dye is used to label the PD-1 antibody, allowing for the detection and analysis of PD-1-expressing cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Cd45ra pe cy5, by BioLegend (1 mentions)
Cd28 pe, by BioLegend (1 mentions)
Il 13 pe, by BioLegend (1 mentions)
Ctla 4 pe, by BioLegend (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Ccr7 pe cy7, by BD (1 mentions)
Anti cd3 apc cy7, by BD (1 mentions)
Cd8 apc, by BD (1 mentions)
Cd8 apc, by BioLegend (1 mentions)
Tnf α fitc, by BioLegend (1 mentions)
Ccr7 pe cy7, by BioLegend (1 mentions)
Anti ifn γ pe, by BioLegend (1 mentions)
Cd45ra pe cy5, by BD (1 mentions)
Anti cd3 apc cy7, by BioLegend (1 mentions)
Cx3cr1 pe, by BioLegend (1 mentions)
Cd27 pe, by BioLegend (1 mentions)
Cytofix cytoperm kit, by BD (1 mentions)
Foxp3 staining buffer set, by BD (1 mentions)
Cd3 pacific orange, by BD (1 mentions)
Cd8 fitc, by BD (1 mentions)
2 protocols using pd 1 pacific blue
1
Comprehensive Immune Cell Profiling
2
Flow Cytometric Profiling of TILs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell surface expression was assessed on freshly isolated TILs or PBMCs by staining with the following monoclonal antibodies: CD3-Pacific Orange (Clone UCHT1, Cat. 561416, BD Biosciences, San Jose, CA, USA), CD4-APC-Cy7 (Clone OKT4, Cat. 317418, Biolegend, San Diego, CA), CD8-FITC (Clone RPA-T8, Cat. 555366, BD Biosciences, San Jose, CA, USA), CD25-Phycoerythrin (Clone M-A251, Cat. 555432, BD Biosciences, San Jose, CA, USA) and PD-1-Pacific Blue (Clone EH12.2H7, Cat. 329916, Biolegend, San Diego, CA). For detection of intracellular expression of FOXP3, cells were permeabilized and fixed with the ‘FOXP3 Staining Buffer Set’ (BD Biosciences, San Jose, CA, USA) following the manufacturer’s instructions, and then they were incubated with the anti-FOXP3-PE-Cy7 monoclonal antibody (Clone PCH101, Ref: 25-4776-42, BD Biosciences, San Jose, CA, USA). Cell analysis was performed with a FACSCanto flow cytometer (BD Biosciences, San Jose, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!