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Flexanalysis version 3.3 software

Manufactured by Bruker

FlexAnalysis (version 3.3) is a software package developed by Bruker for the analysis of data from various analytical instruments. The software provides core functionalities for data processing, visualization, and reporting, without making interpretations or extrapolations about its intended use.

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2 protocols using flexanalysis version 3.3 software

1

MALDI-TOF Mass Spectrometry Protocol

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MALDI-MS
experiments were
carried out on a Bruker Autoflex III MALDI-TOF mass spectrometer (Bruker
Daltonics, Bremen, Germany), equipped with a Smartbeam 2 Nd:YAG laser.
MALDI-MS operating conditions were as follows: ion source 1 = 19.00
kV, ion source 2 = 16.60 kV, lens voltage = 8.44 kV, reflector voltage
= 20.00 kV, reflector voltage 2 = 9.69 kV, and positive reflectron
mode in a mass range of m/z 400–3000.
A total of 200 laser shots was fired per measurement. The laser energy
was optimized to ∼40 μJ/pulse. Data processing was performed
using the Bruker flexAnalysis (version 3.3) software.
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2

Peptide-Sera Interaction Analysis

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Peptide immobilized samples were incubated with 1.0 mL pooled human sera and incubated at 37 °C for 4 hours. At the end of incubation, trifluoroacetic acid was added (0.05%, final concentration) to precipitate plasma protein and release peptides from sample surfaces. Peptides in supernatants were then purified through ZipTipC-18 column (Millipore). The amount of intact peptide was determined using MALDI-TOF mass spectrometry in the matrix containing α-cyano-4-hydrocinnamic acid (10 mg/mL in 50% acetonitrile with 0.05% trifluoroacetic acid). Measurements were made with Bruker UltraFlextreme mass spectrometer and the data analyzed with Flex analysis version 3.3 software (Bruker Daltonik GmbH).
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