Ciprofloxacin

H9C2 and HSkM cell lines, derived from muscle tissues, the epithelial Vero C-76 cells, and the fibroblast (MEF) cells were maintained following the manufacturer's conditions of culture as follows: H9c2 (rat embryonic cardiomyocyte cells) (ATCC® CRL1446 ™) were cultured at 37 °C and 5% CO₂ in Dulbecco's Modified Eagle Medium (DMEM) (Gibco®) supplemented with 10% fetal bovine serum, 100 U. mL⁻¹ penicillin + streptomycin (Gibco®), ciprofloxacin 5 μg.ml⁻¹ (Roemmers®); HSkM (Human Skeletal Muscle cells), generously donated by Dr. George Truskey (University of. Duke, NC, USA), were cultured at 37 °C and 5% CO₂ in DMEM low glucose (Gibco®) supplemented with 10% FBS, 100 U. mL⁻¹ penicillin + streptomycin (Gibco®), ciprofloxacin 5 μg. mL⁻¹ (Roemmers®) and SKGM-SINGLE-QUOT-KIT SUPPL & GROWTH FACTORS (sparing insulin) (Lonza®); Vero C-76 cells (ATCC® TL-1456) (African green monkey kidney cells) were cultured at 37 °C and 5% CO₂ with DMEM (Gibco®) supplemented with 3% FBS, 100 U. mL⁻¹ penicillin + streptomycin (Gibco®), ciprofloxacin 5 μg. mL⁻¹ (Roemmers®); MEF cells (Mouse Embryonic Fibroblasts) were cultured at 37 °C and 5% CO₂ with DMEM supplemented with 3% fetal bovine serum, 100 U. mL⁻¹ penicillin + streptomycin (Gibco®), and ciprofloxacin 5 μg. mL⁻¹ (Roemmers®).