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Xenolight rediject inflammation probe

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The XenoLight RediJect Inflammation Probe is a lab equipment product designed to detect and monitor inflammation in biological samples. It functions by providing a fluorescent signal that indicates the presence and level of inflammation-related markers.

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Lab products found in correlation

Ivis spectrum, by PerkinElmer (8 mentions) Living image software, by PerkinElmer (8 mentions) Living image, by PerkinElmer (3 mentions) Luminol sodium salt, by Merck Group (3 mentions) E coli 0111 b4, by Merck Group (2 mentions) Lumina spectrum, by PerkinElmer (2 mentions) Forane, by Baxter (2 mentions) Lps25, by Merck Group (1 mentions) Living image software v4, by PerkinElmer (1 mentions) Ivis lumina 2 system, by PerkinElmer (1 mentions) Xenogen ivis spectrum in vivo imaging system, by PerkinElmer (1 mentions) Spectrum ct, by PerkinElmer (1 mentions)

21 protocols using xenolight rediject inflammation probe

1

Measuring Inflammation Induced by MSU Crystals

Cited 1 time
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10–12 week-old age and gender matched WT and CD68-POP3 mice were randomly i.p. injected with either PBS (0.5 mL/mouse) or MSU crystals in PBS (10 mg in 0.5 mL PBS/mouse). 5 hrs after MSU injection, mice were i.p. administered the luminescent Xenolight Rediject Inflammation probe (200 mg/kg, PerkinElmer)56 (link). Images were exposed for 5 min (IVIS Spectrum, PerkinElmer) and luminescence quantified with Living Image (PerkinElmer). Mice were also euthanized 7 hrs after MSU injection and peritoneal cavities were flushed with 2 mL of ice-cold PBS/10% FBS, clarified by centrifugation, and analyzed for IL-1β by ELISA.
Khare S., Ratsimandresy R.A., de Almeida L., Cuda C.M., Rellick S.L., Misharin A.V., Wallin M.C., Gangopadhyay A., Forte E., Gottwein E., Perlman H., Reed J.C., Greaves D.R., Dorfleutner A, & Stehlik C. (2014). The PYRIN domain-only protein POP3 inhibits AIM2-like receptor inflammasomes and regulates responses to DNA virus infections. Nature immunology, 15(4), 343-353.
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2

In Vivo Bioluminescence Imaging of Gastrointestinal Inflammation

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Bioluminescence imaging was performed with an IVIS Lumina Spectrum that employs XENOGEN technology (Caliper LifeSciences) under 1.5–2% isoflurane anesthesia. Images of three mice per group were captured and analyzed with Living Image software (Perkin Elmer). The inflammation of the gastrointestinal tract was assessed with the bioluminescent XenoLight RediJect Inflammation Probe (Perkin Elmer), which allows in vivo assessment of myeloperoxidase (MPO) activity. The abdominal region was shaved to reduce the absorption of light. Inflammation Probe (200 mg/kg, around 150 μL) was injected intraperitoneally 4 to 10 min before imaging. The IVIS settings were Epi-BLI, Em filter open, Ex filter block, fstop 1, binning 8, exposure 120 s, and focus B 6.5 cm.
El-Hindi K., Brachtendorf S., Hartel J.C., Oertel S., Birod K., Trautmann S., Thomas D., Ulshöfer T., Weigert A., Utermöhlen O., Krönke M, & Grösch S. (2020). Ceramide Synthase 5 Deficiency Aggravates Dextran Sodium Sulfate-Induced Colitis and Colon Carcinogenesis and Impairs T-Cell Activation. Cancers, 12(7), 1753.
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3

In vivo Inflammation Imaging in Mice

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Eight- to twelve-week-old male WT and CD68-POP2 mice had their abdomen shaved under anaesthesia, and were randomly selected for i.p. injection with PBS, LPS (2.5 mg kg−g, E. coli 0111:B4, #LPS25, Sigma-Aldrich) or MSU crystals (3 mg). After 4 h, mice were i.p. injected with XenoLight Rediject Inflammation probe (200 mg kg−1, #760536, PerkinElmer) or luminol (200 mg kg−1) from a 50 mg ml−1 stock solution of Luminol sodium salt (#A4685, Sigma), dissolved in sterile PBS and stored at −20 °C (ref. 68 (link)) and in vivo bioluminescence was captured by imaging (IVIS Spectrum, PerkinElmer) 10 min post injection with a 5-min exposure on anaesthetized mice13 (link)20 (link). Images were quantified with the Living Image software (PerkinElmer). Peritoneal lavages or blood (mandibular bleed) were collected at the indicated time points after PBS, LPS or MSU injection, and cytokine levels were quantified by ELISA.
Ratsimandresy R.A., Chu L.H., Khare S., de Almeida L., Gangopadhyay A., Indramohan M., Misharin A.V., Greaves D.R., Perlman H., Dorfleutner A, & Stehlik C. (2017). The PYRIN domain-only protein POP2 inhibits inflammasome priming and activation. Nature Communications, 8, 15556.
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4

Quantifying Intestinal Inflammation via Bioluminescence

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Intestinal inflammation was evaluated by XenoLight RediJect Inflammation Probe (PerkinElmer) using an IVIS imaging system (XRMS Serise III). This probe was sensitive to myeloperoxidase production. Specifically, 150 μL of 40 mg/mL probe was injected intraperitoneally 5 min before imaging, and bioluminescence signals in regions of interest were recorded and quantified.
Huang J., Xu Z., Jiao J., Li Z., Li S., Liu Y., Li Z., Qu G., Wu J., Zhao Y., Chen K., Li J., Pan Y., Wu X, & Ren J. (2023). Microfluidic intestinal organoid-on-a-chip uncovers therapeutic targets by recapitulating oxygen dynamics of intestinal IR injury. Bioactive Materials, 30, 1-14.
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5

Inflammation Imaging and Endotoxic Shock

Cited 2 times
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8–12 week old female WT and CD68-POP1 TG mice had their abdomen shaved under anaesthesia, and were randomly selected for i.p. injection with PBS or LPS (2.5 mg/kg, E. coli 0111:B4, Sigma). After 3h, mice were i.p. injected with XenoLight Rediject Inflammation probe (200 mg/kg, PerkinElmer) (Gross et al., 2009 (link)) and in vivo bioluminescence was captured by imaging (IVIS Spectrum, PerkinElmer) 10 min post injection with a 5 min exposure on anesthetized mice (Khare et al., 2014 (link)). Images were quantified with Living Image software (PerkinElmer). Endotoxic shock was induced by i.p. injection of a lethal dose of 20 mg/kg LPS (E. coli 0111:B4) and mice were monitored 4 times daily for survival. Body temperature was measured with an animal rectal probe. Blood was collected 3h post LPS injection by mandibular bleed, and serum cytokine levels were quantified by ELISA.
de Almeida L., Khare S., Misharin A.V., Patel R., Ratsimandresy R.A., Wallin M.C., Perlman H., Greaves D.R., Hoffman H.M., Dorfleutner A, & Stehlik C. (2015). The PYRIN domain-only protein POP1 inhibits inflammasome assembly and ameliorates inflammatory disease. Immunity, 43(2), 264-276.
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6

Myeloperoxidase Activity Quantification

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To determine myeloperoxidase (MPO) activity, DTHA mice were injected i.p. with 150 µl/mouse XenoLight RediJect Inflammation probe (Perkin Elmer), a chemiluminescent substrate of MPO, 1 day after mBSA challenge. Luminescence images were captured by SpectrumCT In Vivo Imaging System (IVIS; Perkin Elmer) 10 min after injection of the probe. The images were acquired with the following parameters (f/stop = 1; exposure time = 180 s; binning factor = 8). The results were expressed as the intensity of radiance (photons/s/cm2/sr) using Living Image software (version 4.2; Perkin Elmer).
Li G., Kolan S.S., Guo S., Marciniak K., Kolan P., Malachin G., Grimolizzi F., Haraldsen G, & Skålhegg B.S. (2021). Activated, Pro-Inflammatory Th1, Th17, and Memory CD4+ T Cells and B Cells Are Involved in Delayed-Type Hypersensitivity Arthritis (DTHA) Inflammation and Paw Swelling in Mice. Frontiers in Immunology, 12, 689057.
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7

Measuring Inflammation Induced by MSU Crystals

Cited 1 time
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10–12 week-old age and gender matched WT and CD68-POP3 mice were randomly i.p. injected with either PBS (0.5 mL/mouse) or MSU crystals in PBS (10 mg in 0.5 mL PBS/mouse). 5 hrs after MSU injection, mice were i.p. administered the luminescent Xenolight Rediject Inflammation probe (200 mg/kg, PerkinElmer)56 (link). Images were exposed for 5 min (IVIS Spectrum, PerkinElmer) and luminescence quantified with Living Image (PerkinElmer). Mice were also euthanized 7 hrs after MSU injection and peritoneal cavities were flushed with 2 mL of ice-cold PBS/10% FBS, clarified by centrifugation, and analyzed for IL-1β by ELISA.
Khare S., Ratsimandresy R.A., de Almeida L., Cuda C.M., Rellick S.L., Misharin A.V., Wallin M.C., Gangopadhyay A., Forte E., Gottwein E., Perlman H., Reed J.C., Greaves D.R., Dorfleutner A, & Stehlik C. (2014). The PYRIN domain-only protein POP3 inhibits AIM2-like receptor inflammasomes and regulates responses to DNA virus infections. Nature immunology, 15(4), 343-353.
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8

Measuring Inflammatory Response in Mice

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8- to 12-week-old WT and POP1TG mice were injected with 20 μl PBS or MSU crystals (100 μg/ml) using a Hamilton syringe into the left and right hind limb ankle joint and 24h later, injected i.p. with XenoLight Rediject Inflammation probe (200 mg/kg, PerkinElmer) and imaged as above. Ankle circumference was measured with a caliper before injection and 24 h after injection.
de Almeida L., Devi S., Indramohan M., Huang Q.Q., Ratsimandresy R.A., Pope R.M., Dorfleutner A, & Stehlik C. (2022). POP1 inhibits MSU-induced inflammasome activation and ameliorates gout. Frontiers in Immunology, 13, 912069.
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9

Inflammatory Response to MSU Crystals in Air Pouch Model

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8- to 12-week-old WT and POP1TG mice had their back shaved under anesthesia and 3 ml of sterile air was injected subcutaneously on the back to generate an air pouch using a 25-gauge syringe. Three days later, subcutaneous air pouches were re-inflated with 2 ml of sterile air. Mice were randomly selected for subcutaneous air pouch injection with PBS or MSU crystals (3 mg) on day 6. After 6h, mice were injected i.p. with a XenoLight Rediject Inflammation probe (200 mg/kg, PerkinElmer) and in vivo bioluminescence was captured by imaging (IVIS Spectrum, PerkinElmer) 10 min after injection with a 5 min exposure time on anesthetized mice. Images were quantified with Living Image software (PerkinElmer, RRID : SCR_014247). Subcutaneous air pouch lavage exudates were collected 6h after MSU crystal injection. Cells were recovered from the lavage exudate and were analyzed by flow cytometry and cytokine levels were quantified by ELISA.
de Almeida L., Devi S., Indramohan M., Huang Q.Q., Ratsimandresy R.A., Pope R.M., Dorfleutner A, & Stehlik C. (2022). POP1 inhibits MSU-induced inflammasome activation and ameliorates gout. Frontiers in Immunology, 13, 912069.
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10

Bioluminescence Imaging of Myeloperoxidase Activity in EAE

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In vivo imaging was performed with an IVIS Lumina Spectrum, which allows for the analysis of bioluminescence and near-infrared signals, and was analyzed with LivingImage software (PerkinElmer). Four 8, 11, and 15 days after EAE immunization, 150 μl of XenoLight RediJect Inflammation Probe (40 mg/ml; PerkinElmer), which recognizes myeloperoxidase activity, was injected intraperitoneally. Bioluminescence was captured 5, 10, and 15 min after injection in 9–10 animals per genotype to assess individual differences in XenoLight kinetics. During imaging, mice were kept under 1–1.5% isoflurane anesthesia. The IVIS settings were as follows: Epi-bioluminescence, emission filter open, excitation filter block, fstop = 1, binning = 8, focus B = 6.5 cm, exposure = 60 sec. The regions of interest (ROI) i.e., the injection sites, were identified by using the automatic detection tool in LivingImage. For each mouse, the two time points with the maximum total counts of bioluminescence in the ROIs were used to statistically analyze the genotype differences.
Schmitz K., Wilken-Schmitz A., Vasic V., Brunkhorst R., Schmidt M, & Tegeder I. (2019). Progranulin deficiency confers resistance to autoimmune encephalomyelitis in mice. Cellular and Molecular Immunology, 17(10), 1077-1091.
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