Nod cb17 prkdcscid j

Male and female NOD/ShiLtJ (NOD), NOD.CB17-Prkdc^scid/J (NOD-SCID), NOD.129S6(B6)-CD4^tm1knw/DvsJ (CD4^null), and Balb/cJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Male and female NOD.129S2(B6)-CD8a^tm1Mak/DvsJ (CD8^null) mice were kindly provided by David Serreze (The Jackson Laboratory). NOD mice expressing the bicistronic Foxp3-green fluorescent protein (GFP) (NOD), obtained by backcrossing the Foxp3-GFP knock-in C57BL/6J mice^{56 (link)} for > 20 generations onto the NOD background, were a kind gift from Vijay Kuchroo (Harvard University). For transfer studies, donor mice were 5–6 weeks old and recipients were 7–9 weeks old. For ex vivo analyses, mice were 12–17 weeks old. The exact sample size (n) and biological and experimental replicates are indicated in each figure legend. Samples sizes were determined based on previous transfer studies and typically included 10–15 mice per group pooled from multiple experiments. All mice were monitored for the presence of glucosuria using Diastix urine dipsticks (Bayer Diagnostics, Whippany, NJ). A positive test was indicative of autoimmune diabetes development. Mice were maintained and used in accordance with the University of Iowa Institutional Animal Care and Use Committee Guidelines.

Animal research was conducted in compliance with the Institutional Animal Care and Use Committees at Massachusetts General Hospital (MGH). Unless otherwise stated, experiments were performed using female C57BL/6 that were 6- to 8-weeks old at the start of the experiment, and animals were sourced from The Jackson Laboratory. Intravital examination of CDNP-VT680 distribution into macrophages was examined in recently developed NOD MerTK^GFP/+ mice⁴⁵ , crossed into NOD SCID mice (NOD.CB17-Prkdcscid/J; #001303, Jackson). Intravital examination of IL12 expression was performed using p40-IRES-eYFP IL12 reporter mice (#015864, Jackson) described previously^{70 (link)}.